{Reference Type}: Journal Article
{Title}: Using Methylation-Specific PCR to Study RB1 Promoter Hypermethylation.
{Author}: McCormick TM;Carvalho MDGDC;
{Journal}: Methods Mol Biol
{Volume}: 1726
{Issue}: 0
{Year}: 2018
暂无{DOI}: 10.1007/978-1-4939-7565-5_4
{Abstract}: It has increasingly been considered crucial the understanding of DNA methylation of Tumor Suppressor Gene (TSG) promoters, such as that of retinoblastoma 1 gene (RB1), and its role during carcinogenesis. We present a detailed and optimized protocol of the methylation-specific PCR (MSP) technique to study RB1 gene promoter hypermethylation.