Abstract：
Knowledge on baseline values of stress biomarkers in natural conditions is urgent due to the need of reference values for monitoring purposes. Here we assessed the cellular stress response of the chiton Chaetopleura angulata in situ. Biomarkers commonly used in environmental monitoring (heat shock protein 70kDa, total ubiquitin, catalase, glutathione-S-transferase, superoxide-dismutase, lipid peroxidation) were analyzed in the digestive system, gills and muscle of C. angulata, under spring and summer conditions in order to assess seasonal tissue-specific responses. Season had an effect on all targeted organs, especially affecting the digestive system which displayed clear seasonal clusters. The respective Integrated Biomarker Response (IBR) showed a 7.2-fold seasonal difference. Muscle and gills showed similar IBRs between seasons making them appropriate organs to monitor chemical pollution as they were less responsive to seasonal variation. The most stable biomarkers in these organs were ubiquitin and superoxide-dismutase thus being reliable for monitoring purposes.
摘要：
由于需要用于监测目的的参考值，因此迫切需要了解自然条件下应激生物标志物的基线值。在这里，我们原位评估了ChitonChaetopleuraangulata的细胞应激反应。环境监测中常用的生物标志物(热休克蛋白70kDa，总泛素，过氧化氢酶,谷胱甘肽-S-转移酶，超氧化物歧化酶,脂质过氧化)在消化系统中进行分析，角C.g和肌肉，在春季和夏季条件下，以评估季节性组织特异性反应。季节对所有目标器官都有影响，特别是影响消化系统，表现出明显的季节性集群。各自的综合生物标志物响应(IBR)显示7.2倍的季节性差异。肌肉和g在季节之间显示出相似的IBR，这使它们成为监测化学污染的合适器官，因为它们对季节变化的反应较小。这些器官中最稳定的生物标志物是泛素和超氧化物歧化酶，因此对于监测目的是可靠的。
