关键词: BOX-PCR Escherichia coli Fecal pollution Gulls Microbial source tracking Public health

Mesh : Animals Charadriiformes / physiology Environmental Monitoring Escherichia coli / growth & development isolation & purification Feces Portugal Water Microbiology Water Pollution / analysis statistics & numerical data

来  源:   DOI:10.1016/j.scitotenv.2013.09.075   PDF(Sci-hub)

Abstract:
Gulls were reported as sources of fecal pollution in coastal environments and potential vectors of human infections. Microbial source tracking (MST) methods were rarely tested to identify this pollution origin. This study was conducted to ascertain the source of water fecal contamination in the Berlenga Island, Portugal. A total of 169 Escherichia coli isolates from human sewage, 423 isolates from gull feces and 334 water isolates were analyzed by BOX-PCR. An average correct classification of 79.3% was achieved. When an 85% similarity cutoff was applied 24% of water isolates were present in gull feces against 2.7% detected in sewage. Jackknifing resulted in 29.3% of water isolates classified as gull, and 10.8% classified as human. Results indicate that gulls constitute a major source of water contamination in the Berlenga Island. This study validated a methodology to differentiate human and gull fecal pollution sources in a real case of a contaminated beach.
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