Thiobencarb herbicide reduces growth, photosynthetic activity, and amount of Rieske iron-sulfur protein in the diatom Thalassiosira pseudonana.

• 影响指数: 3.568
• 发表时间: Sep 2013
• 来源期刊: J Biochem Mol Toxicol
• DOI: 10.1002/jbt.21505
• PMID: 23801610
• 文章类型: Journal Article
• 中科院分区: Q3
• 方向: 生物
• 作者列表: Shimasaki Y,Tsuyama M,Tasmin R,Qiu X,Shimizu M,Sato Y,Yamasaki Y,Kato-Unoki Y,Nukata A,Nakashima T,Ichinose H,Wariishi H,Honjo T,Oshima Y

关键词: Proteomics; Rieske Iron-Sulfur Protein; Thalassiosira pseudonana; Thiobencarb

Mesh: Amino Acid Sequence; Diatoms / drug effects; Electron Transport Complex III / metabolism; Herbicides / pharmacology; Oxidation-Reduction; Photosynthesis / drug effects; Thiocarbamates / pharmacology

Abstract

We investigated the effects of the herbicide thiobencarb on the growth, photosynthetic activity, and expression profile of photosynthesis-related proteins in the marine diatom Thalassiosira pseudonana. Growth rate was suppressed by 50% at a thiobencarb concentration of 1.26 mg/L. Growth and photosystem II activity (Fv /Fm ratio) were drastically decreased at 5 mg/L, at which the expression levels of 13 proteins increased significantly and those of 11 proteins decreased significantly. Among these proteins, the level of the Rieske iron-sulfur protein was decreased to less than half of the control level. This protein is an essential component of the cytochrome b6 f complex in the photosynthetic electron transport chain. Although the mechanism by which thiobencarb decreased the Rieske iron-sulfur protein level is not clear, these results suggest that growth was inhibited by interruption of the photosynthetic electron transport chain by thiobencarb.