%0 Journal Article
%T Thiobencarb herbicide reduces growth, photosynthetic activity, and amount of Rieske iron-sulfur protein in the diatom Thalassiosira pseudonana.
%A Shimasaki Y
%A Tsuyama M
%A Tasmin R
%A Qiu X
%A Shimizu M
%A Sato Y
%A Yamasaki Y
%A Kato-Unoki Y
%A Nukata A
%A Nakashima T
%A Ichinose H
%A Wariishi H
%A Honjo T
%A Oshima Y
%J J Biochem Mol Toxicol
%V 27
%N 9
%D Sep 2013
%M 23801610
%F 3.568
%R 10.1002/jbt.21505
%X We investigated the effects of the herbicide thiobencarb on the growth, photosynthetic activity, and expression profile of photosynthesis-related proteins in the marine diatom Thalassiosira pseudonana. Growth rate was suppressed by 50% at a thiobencarb concentration of 1.26 mg/L. Growth and photosystem II activity (Fv /Fm ratio) were drastically decreased at 5 mg/L, at which the expression levels of 13 proteins increased significantly and those of 11 proteins decreased significantly. Among these proteins, the level of the Rieske iron-sulfur protein was decreased to less than half of the control level. This protein is an essential component of the cytochrome b6 f complex in the photosynthetic electron transport chain. Although the mechanism by which thiobencarb decreased the Rieske iron-sulfur protein level is not clear, these results suggest that growth was inhibited by interruption of the photosynthetic electron transport chain by thiobencarb.