Abstract：
Dental caries is one of the most common diseases affecting more than 2 billion people\'s health worldwide. In a clinical setting, it is challenging to predict and proactively guard against dental cavities prior to receiving a confirmed diagnosis. Streptococcus mutans (S. mutans) in saliva has been recognized as the main causative bacterial agent that causes dental caries. High sensitivity, good selectivity, and a wide detection range are incredibly important factors to affect S. mutans detection in practical applications. In this study, we present a portable saliva biosensor designed for the early detection of S. mutans with the potential to predict the occurrence of dental cavities. The biosensor was fabricated using a S. mutans-specific DNA aptamer and S. mutans-imprinted polymers. Methylene blue was utilized as a redox probe in the sensor to generate current signals for analysis. When S. mutans enters complementarily S. mutans cavities, it blocks electron transfer between methylene blue and the electrode, resulting in decreases in the reduction current signal. The signal variations are associated with S. mutans concentrations that are useful for quantitative analysis. The linear detection range of S. mutans is 102-109 cfu mL-1, which covers the critical concentration of high caries risk. The biosensor exhibited excellent selectivity toward S. mutans in the presence of other common oral bacteria. The biosensor\'s wide detection range, excellent selectivity, and low limit of detection (2.6 cfu mL-1) are attributed to the synergistic effect of aptamer and S. mutans-imprinted polymers. The sensor demonstrates the potential to prevent dental caries.
摘要：
龋齿是影响全球超过20亿人健康的最常见疾病之一。在临床环境中，在接受确诊诊断之前,预测并主动防范牙洞是一项挑战.变形链球菌(S.唾液中的mutans）已被认为是引起龋齿的主要致病菌。灵敏度高,良好的选择性，在实际应用中，广泛的检测范围是影响变形链球菌检测的重要因素。在这项研究中,我们提出了一种便携式唾液生物传感器，设计用于早期检测变形链球菌，有可能预测牙洞的发生。使用变形链球菌特异性DNA适体和变形链球菌印迹聚合物制造生物传感器。亚甲基蓝用作传感器中的氧化还原探针以产生用于分析的电流信号。当变形链球菌进入互补的变形链球菌空腔时，它阻止亚甲蓝和电极之间的电子转移，导致减少电流信号。信号变化与可用于定量分析的变形链球菌浓度相关。变形链球菌的线性检测范围为102-109cfumL-1，涵盖了高龋齿风险的临界浓度。在其他常见口腔细菌的存在下，生物传感器对变形链球菌表现出优异的选择性。生物传感器检测范围广,优异的选择性，和低检测限（2.6cfumL-1）归因于适体和变形链球菌印迹聚合物的协同作用。该传感器显示出预防龋齿的潜力。
