玉米赤霉烯酮(ZEN)是引起家畜生殖毒性的雌激素性霉菌毒素。目前,内酯水解酶用于ZEN的酶促降解。然而,大多数内酯水解酶的降解效率低,热稳定性差。ZHD518,作为ZEN降解的中性酶,在中性条件下表现出高的酶活性。在这项研究中,将多功能肽S1v1-(AEAEAHAH)2融合至ZHD518的N末端。与野生型酶相比,肽融合蛋白表达显著增强1.28倍,酶活性提高了9.27倍,在45°C孵育10分钟后,热稳定性提高了37.08倍,并且在长期储存期间酶具有稳定性。此外,玉米麸皮中的ZEN浓度,玉米胚芽粉,玉米蛋白粉从5.29±0.04,5.31±0.03,5.30±0.01μg/g下降到0.48±0.05,0.48±0.06,0.21±0.04μg/g,分别,用S1v1-GS-ZHD518处理60分钟后,降解率为90.98、91.00和95.32%,分别。总之,S1v1-GS-ZHD518的性能,如高效降解,耐高温和耐储存,提供其在食品或饲料中应用的可能性。
Zearalenone (ZEN) is an estrogenic mycotoxin causing reproductive toxicity in livestock. Currently, lactone hydrolases are used in the enzymatic degradation of ZEN. However, most lactone hydrolases suffer from low degradation efficiency and poor thermal stability. ZHD518, as a documented neutral enzyme for ZEN degradation, exhibits high enzymatic activity under neutral conditions. In this study, a multifunctional peptide S1v1-(AEAEAHAH)2 was fused to the N-terminus of ZHD518. Compared with the wild-type enzyme, the peptide fusion significantly enhanced protein expression by 1.28 times, enzyme activity by 9.27 times, thermal stability by 37.08 times after incubation at 45 °C for 10 min and enzyme stability during long-term storage. Moreover, ZEN concentrations in corn bran, corn germ meal, and corn gluten powder decreased from 5.29 ± 0.04, 5.31 ± 0.03, and 5.30 ± 0.01 μg/g to 0.48 ± 0.05, 0.48 ± 0.06, and 0.21 ± 0.04 μg/g, respectively, following a 60 min treatment with S1v1-GS-ZHD518, resulting in degradation rates of 90.98, 91.00, and 95.32%, respectively. In conclusion, the properties of S1v1-GS-ZHD518, such as its efficient degradability, high temperature resistance and storage resistance, offer the possibility of its application in food or feed.