糖尿病伤口是严重的临床并发症,由于大量渗出物而表现为湿润状态,随着炎症的调节受到干扰,严重的氧化应激和反复的细菌感染。由于缺乏包含机械性能的理想敷料,现有的糖尿病伤口治疗方法仍不令人满意。粘附在潮湿的组织表面,快速修复,和不同的治疗益处。在这里,我们制造了一种湿粘合剂,自我修复,具有有效抗菌和促愈合特性的葡萄糖响应药物释放水凝胶,用于糖尿病伤口治疗。PAE水凝胶是用聚(丙烯酸-共-丙烯酰胺)(AA-Am)与动态E-F交联剂集成构建的,由表没食子儿茶素没食子酸酯(EGCG)和4-(2-丙烯酰胺基乙基氨基甲酰基)-3-氟苯基硼酸(AFPBA)组成。由于硼酸酯的动态交联性质,丰富的邻苯二酚基团和氢键,PAE水凝胶表现出优异的机械性能,伸长率约为1000%,对潮湿组织的牢固粘附,快速自我修复,和吸收自身重量10倍的生物流体。重要的是,PAE水凝胶显示EGCG的持续和葡萄糖响应性释放。一起,生物活性PAE水凝胶具有有效的抗菌作用,抗氧化,和体外抗炎特性,并通过减少组织炎症反应加速大鼠的糖尿病伤口愈合,增强血管生成,和巨噬细胞的重编程。总的来说,这种多功能水凝胶为糖尿病伤口的治疗提供了一个简单的解决方案,并显示了其他伤口相关应用场景的潜力。
Diabetic wounds are serious clinical complications which manifest wet condition due to the mass exudate, along with disturbed regulation of inflammation, severe oxidative stress and repetitive bacterial infection. Existing treatments for diabetic wounds remain unsatisfactory due to the lack of ideal dressings that encompass mechanical performance, adherence to moist tissue surfaces, quick repair, and diverse therapeutic benefits. Herein, we fabricated a wet adhesive, self-healing, glucose-responsive drug releasing hydrogel with efficient antimicrobial and pro-healing properties for diabetic wound treatment. PAE hydrogel was constructed with poly(acrylic acid-co-acrylamide) (AA-Am) integrated with a dynamic E-F crosslinker, which consisted of epigallocatechin gallate (EGCG) and 4-(2-acrylamidoethylcarbamoyl)-3-fluorophenylboronic acid (AFPBA). Due to the dynamic crosslinking nature of boronate esters, abundant catechol groups and hydrogen bonding, PAE hydrogel demonstrated excellent mechanical properties with about 1000 % elongation, robust adhesion to moist tissues, fast self-healing, and absorption of biofluids of 10 times of its own weight. Importantly, PAE hydrogel exhibited sustained and glucose-responsive release of EGCG. Together, the bioactive PAE hydrogel had effective antibacterial, antioxidative, and anti-inflammatory properties in vitro, and accelerated diabetic wound healing in rats via reducing tissue-inflammatory response, enhancing angiogenesis, and reprogramming of macrophages. Overall, this versatile hydrogel provides a straightforward solution for the treatment of diabetic wound, and shows potential for other wound-related application scenarios.