断奶是仔猪生长发育的关键阶段,经常引起应激反应。本研究旨在研究宁乡猪来源的双反杆菌属(PBd)对其生长性能的影响,肠道细胞凋亡,氧化损伤,和ETEC攻击的断奶仔猪的炎症。共有22只杜洛克×长白猪×约克郡(DLY)仔猪,24天大体重相似,随机分为三组:对照组(n=7),ETEC(n=7),和PBd+ETEC(n=8)。结果表明,与对照组相比,ETEC挑战导致增长业绩下降,十二指肠和空肠绒毛高度降低,十二指肠隐窝深度增加,绒毛高度与隐窝深度之比降低,凋亡相关基因(Caspase-8和Caspase-9)的表达增加,氧化损伤相关基因的表达增加(Nrf2,GSH-PX,mTOR,和Beclin1),炎症相关基因表达增加(Myd88,P65,TNF-α,和IL-6),并降低了结肠食糜中SCFA的含量(乙酸盐,丙酸盐,丁酸盐,戊酸盐,和总SCFA)。与ETEC组相比,PBd+ETEC集团缓解了增长业绩的下降,减轻肠道形态损伤,并减少上述细胞凋亡的表达,氧化损伤,和炎症相关基因随着SCFA的增加。总之,宁乡猪来源的PBd能有效降低ETEC诱导的断奶仔猪肠道损伤,改善肠道健康,并增加结肠食糜中SCFA的含量,从而提高增长绩效。
Weaning is a critical stage in the growth and development of piglets, often inducing stress reactions. This study aims to investigate the effects of Parabacteroides distasonis (PBd) derived from Ningxiang pigs on growth performance, intestinal apoptosis, oxidative damage, and inflammation in ETEC-challenged weaned piglets. A total of 22 Duroc × Landrace × Yorkshire (DLY) piglets, 24 days old with similar body weights, were randomly divided into three groups: Control (n = 7), ETEC (n = 7), and PBd + ETEC (n = 8). The results show that, compared to the Control group, ETEC challenge led to decreased growth performance, reduced villus height in the duodenum and jejunum, increased crypt depth in the duodenum, a decreased villus-height-to-crypt-depth ratio, increased expression of apoptosis-related genes (Caspase-8 and Caspase-9), increased expression of oxidative damage-related genes (Nrf2, GSH-PX, mTOR, and Beclin1), increased expression of inflammation-related genes (Myd88, P65, TNF-α, and IL-6), and reduced the contents of SCFAs in the colonic chyme (acetate, propionate, butyrate, valerate, and total SCFAs). Compared to the ETEC group, the PBd + ETEC group alleviated the reduction in growth performance, mitigated intestinal morphological damage, and reduced the expression of the aforementioned apoptosis, oxidative damage, and inflammation-related genes with the increase in SCFAs. In conclusion, PBd derived from Ningxiang pigs effectively reduces ETEC-induced intestinal damage in weaned piglets, improves intestinal health, and increases the content of SCFAs in the colonic chyme, thereby enhancing growth performance.