根据内部转录间隔物(ITS),从海胆Chaphechinusmirabilis中分离出的海洋真菌菌株KMM4718和KMM4747作为天然真菌复合物被鉴定为sajarovii青霉和隆曲霉,部分β-微管蛋白(BenA),和钙调蛋白(CaM)分子标记以及核糖体聚合酶两种,二亚基(RPB2)区为4747KMM。从共培养物的乙酸乙酯提取物中,两种新的聚酮化合物,sajaroketidesA(1)和B(2),连同(2'S)-7-羟基-2-(2'-羟丙基)-5-甲基色酮(3),altchromoneA(4),norlichexanthone(5),灰黄酮C(6),1,3,5,6-四羟基-8-甲基黄原酮(7),灰黄霉素(8),6-O-去甲基灰黄霉素(9),dechlorogriseofulvin(10),和5,6-二氢-4-甲基-2H-吡喃-2-酮(11)被鉴定。使用光谱分析阐明化合物的结构。使用基于时间依赖性密度泛函理论(TDDFT)的电子圆二色性(ECD)光谱计算确定了sajaroketidesA和B的手性中心的绝对构型。这些化合物对脲酶活性和金黄色葡萄球菌生长的抑制作用,大肠杆菌,观察到白色念珠菌。SajaroketideA,altechromoneA,和灰黄霉素在金黄色葡萄球菌诱导的感染性心肌炎的体外模型中显示出显著的心脏保护作用。
The marine-derived fungal strains KMM 4718 and KMM 4747 isolated from sea urchin Scaphechinus mirabilis as a natural fungal complex were identified as Penicillium sajarovii and Aspergillus protuberus based on Internal Transcribed Spacer (ITS), partial β-tubulin (BenA), and calmodulin (CaM) molecular markers as well as an ribosomal polymerase two, subunit two (RPB2) region for KMM 4747. From the ethyl acetate extract of the co-culture, two new polyketides, sajaroketides A (1) and B (2), together with (2\'S)-7-hydroxy-2-(2\'-hydroxypropyl)-5-methylchromone (3), altechromone A (4), norlichexanthone (5), griseoxanthone C (6), 1,3,5,6-tetrahydroxy-8-methylxanthone (7), griseofulvin (8), 6-O-desmethylgriseofulvin (9), dechlorogriseofulvin (10), and 5,6-dihydro-4-methyl-2H-pyran-2-one (11) were identified. The structures of the compounds were elucidated using spectroscopic analyses. The absolute configurations of the chiral centers of sajaroketides A and B were determined using time-dependent density functional theory (TDDFT)-based calculations of the Electronic Circular Dichroism (ECD) spectra. The inhibitory effects of these compounds on urease activity and the growth of Staphylococcus aureus, Escherichia coli, and Candida albicans were observed. Sajaroketide A, altechromone A, and griseofulvin showed significant cardioprotective effects in an in vitro model of S. aureus-induced infectious myocarditis.