背景:由于其多样化的生物活性,来自银耳的多糖已经受到越来越多的关注。本研究旨在研究银耳来源的纯化多糖(称为PTP-3a)的结构表征,并探索其与肠道菌群的体外相互作用。
结果:研究结果表明,PTP-3a的分子量为1.22×103kDa,由岩藻糖组成,葡萄糖,木糖,甘露糖,和葡萄糖醛酸的摩尔比为0.271:0.016:0.275:0.400:0.038。在PTP-3a中鉴定的主要连锁类型是1,3-连接-manp,1,4-连接-xylp,和1,2,3-链接-fucp,相应的比率为0.215:0.161:0.15。此外,PTP-3a表现出显著的热稳定性并表现出三螺旋结构。此外,在体外发酵48小时后,PTP-3a得到有效利用,导致碳水化合物水平降低,短链脂肪酸(SCFA)的生产,和pH调节。此外,在体外粪便微生物发酵过程中,PTP-3a降低了厚壁菌的相对丰度,同时增加了拟杆菌和变形杆菌的比例,导致Firmicutes/拟杆菌比率显着降低。此外,PTP-3a刺激有益菌的生长,如MerdaeGordonibacterpamelaeae,双歧杆菌,和分布式副杆菌属。重要的是,在SCFA的产生与特定微生物之间观察到很强的相关性。
结论:这些发现表明PTP-3a具有作为调节肠道微生物群的益生元的潜力。本文受版权保护。保留所有权利。
BACKGROUND: Because of their diverse biological activities, polysaccharides derived from Tremella fuciformis have received growing attention. This study aimed to investigate the structural characterization of a purified polysaccharide (designated as PTP-3a) derived from T. fuciformis and explore its interaction with gut microbiota in vitro.
RESULTS: The findings revealed that PTP-3a had a molecular weight of 1.22 × 103 kDa and consisted of fucose, glucose, xylose, mannose and glucuronic acid in a molar ratio of 0.271:0.016:0.275:0.400:0.038. The primary linkage types identified in PTP-3a were 1,3-linked-manp, 1,4-linked-xylp and 1,2,3-linked-fucp, with corresponding ratios of 0.215:0.161:0.15. In addition, PTP-3a demonstrated notable thermal stability and exhibited a triple-helical structure. Moreover, following in vitro fermentation for 48 h, PTP-3a was efficiently utilized, resulting in a reduction in carbohydrate levels, the production of short-chain fatty acids (SCFAs) and pH adjustment. Furthermore, during in vitro fecal microbial fermentation, PTP-3a decreased the relative abundance of Firmicutes while increasing the proportions of Bacteroidetes and Proteobacteria, resulting in a significantly reduced Firmicutes/Bacteroidetes ratio. Additionally, PTP-3a stimulated the growth of beneficial bacteria such as Parabacteroides merdae, Gordonibacter pamelaeae, Bifidobacterium pseudolongum and Parabacteroides distasonis. Importantly, a strong correlation was observed between the production of SCFAs and specific microorganisms.
CONCLUSIONS: These findings suggested that PTP-3a has potential as a prebiotic for modulating the gut microbiota. © 2024 Society of Chemical Industry.