钩端螺旋体病,马来西亚的一种需报告的地方病,死亡率高于区域性登革热。不同的临床症状和有限的诊断方法使钩端螺旋体病诊断复杂化。对准确的基于生物标志物的诊断的需求正在增加。这项研究使用等量异位标签进行相对和绝对定量(iTRAQ)-质谱(MS),与登革热患者和健康受试者相比,调查了钩端螺旋体血症和血清转换的钩端螺旋体病患者的血浆蛋白质组。iTRAQ分析总共鉴定出450种蛋白质,通过一系列排除标准将其细化为290种蛋白质。与对照组相比,钩端螺旋体病患者血浆蛋白质组中的差异表达鉴定出11种蛋白质,它们是载脂蛋白A-II(APOA2),C反应蛋白(CRP),铁蛋白家族同源物3(FERMT3),富含亮氨酸的α-2-糖蛋白1(LRG1),脂多糖结合蛋白(LBP),肌球蛋白-9(MYH9),血小板碱性蛋白(PPBP),血小板因子4(PF4),profilin-1(PFN1),血清淀粉样蛋白A-1(SAA1),和血小板反应蛋白-1(THBS1)。在对一个验证队列进行研究之后,一组八个血浆蛋白生物标志物被鉴定为潜在的钩端螺旋体病诊断:CRP,LRG1,LBP,MYH9,PPBP,PF4、SAA1和THBS1。总之,一组八种蛋白质生物标志物为钩端螺旋体病的诊断提供了一种有希望的方法,解决“一种疾病”的局限性,一种生物标志物“概念。
Leptospirosis, a notifiable endemic disease in Malaysia, has higher mortality rates than regional dengue fever. Diverse clinical symptoms and limited diagnostic methods complicate leptospirosis diagnosis. The demand for accurate biomarker-based diagnostics is increasing. This study investigated the plasma proteome of leptospirosis patients with leptospiraemia and seroconversion compared with dengue patients and healthy subjects using isobaric tags for relative and absolute quantitation (iTRAQ)-mass spectrometry (MS). The iTRAQ analysis identified a total of 450 proteins, which were refined to a list of 290 proteins through a series of exclusion criteria. Differential expression in the plasma proteome of leptospirosis patients compared to the control groups identified 11 proteins, which are apolipoprotein A-II (APOA2), C-reactive protein (CRP), fermitin family homolog 3 (FERMT3), leucine-rich alpha-2-glycoprotein 1 (LRG1), lipopolysaccharide-binding protein (LBP), myosin-9 (MYH9), platelet basic protein (PPBP), platelet factor 4 (PF4), profilin-1 (PFN1), serum amyloid A-1 protein (SAA1), and thrombospondin-1 (THBS1). Following a study on a verification cohort, a panel of eight plasma protein biomarkers was identified for potential leptospirosis diagnosis: CRP, LRG1, LBP, MYH9, PPBP, PF4, SAA1, and THBS1. In conclusion, a panel of eight protein biomarkers offers a promising approach for leptospirosis diagnosis, addressing the limitations of the \"one disease, one biomarker\" concept.