由于细菌感染,发病率和死亡率不断上升,特别是金黄色葡萄球菌有必要寻找解决方案来面对这个问题。因此,我们阐明了Cleomedroserifolia提取物(CDE)的植物化学成分和抗菌潜力。使用LC-ESI-MS/MS,探索了CDE的主要植物成分,是山奈酚-3,7-O-双-α-L-鼠李糖苷,异鼠李素,花青素-3-葡萄糖苷,Kaempferide,山奈酚-3-O-α-L-鼠李糖苷,咖啡酸,异槲皮苷,奎尼酸,异柠檬酸,甘露醇,芹菜素,acacetin,还有Naringenin.CDE对金黄色葡萄球菌分离株具有抗菌作用,最小抑制浓度范围为128至512µg/mL。此外,使用结晶紫测定法,CDE表现出抗生物膜作用。使用扫描电子显微镜来阐明CDE对生物膜形成的影响,它大大减少了生物膜中的金黄色葡萄球菌细胞数量。此外,进行qRT-PCR以研究CDE对生物膜基因表达的影响(cna,fnba,和icaA)。CDE揭示了在43.48%的金黄色葡萄球菌分离物中对所研究的生物膜基因的下调作用。关于体内模型,CDE显著降低CDE处理小鼠肝脏和脾脏中的金黄色葡萄球菌负荷。此外,它显著提高了小鼠的存活率,并显著降低了所研究组织中的炎症标志物(白细胞介素一β和白细胞介素六)。此外,CDE改善了CDE治疗组肝脏和脾脏的组织学和肿瘤坏死因子α免疫组织化学。因此,CDE可以被认为是未来抗微生物药物发现研究的有希望的候选者。
The increasing rates of morbidity and mortality owing to bacterial infections, particularly Staphylococcus aureus have necessitated finding solutions to face this issue. Thus, we elucidated the phytochemical constituents and antibacterial potential of Cleome droserifolia extract (CDE). Using LC-ESI-MS/MS, the main phytoconstituents of CDE were explored, which were kaempferol-3,7-O-bis-alpha-L-rhamnoside, isorhamnetin, cyanidin-3-glucoside, kaempferide, kaempferol-3-O-alpha-L-rhamnoside, caffeic acid, isoquercitrin, quinic acid, isocitrate, mannitol, apigenin, acacetin, and naringenin. The CDE exerted an antibacterial action on S. aureus isolates with minimum inhibitory concentrations ranging from 128 to 512 µg/mL. Also, CDE exhibited antibiofilm action using a crystal violet assay. A scanning electron microscope was employed to illuminate the effect of CDE on biofilm formation, and it considerably diminished S. aureus cell number in the biofilm. Moreover, qRT-PCR was performed to study the effect of CDE on biofilm gene expression (cna, fnbA, and icaA). The CDE revealed a downregulating effect on the studied biofilm genes in 43.48% of S. aureus isolates. Regarding the in vivo model, CDE significantly decreased the S. aureus burden in the liver and spleen of CDE-treated mice. Also, it significantly improved the mice\'s survival and substantially decreased the inflammatory markers (interleukin one beta and interleukin six) in the studied tissues. Furthermore, CDE has improved the histology and tumor necrosis factor alpha immunohistochemistry in the liver and spleen of the CDE-treated group. Thus, CDE could be considered a promising candidate for future antimicrobial drug discovery studies.