GAI-RGA-and-SCR(GRAS)转录因子可以调节植物生长发育和逆境防御等多种生物过程,但有关甜菜的研究很少。盐胁迫可严重影响甜菜的产量和品质。因此,本研究采用生物信息学方法对甜菜中的GRAS转录因子进行鉴定,并分析其结构特征,进化关系,调节网络和盐应激反应模式。在甜菜全基因组中共鉴定出28个BvGRAS基因,序列组成相对保守。根据系统发育树的拓扑结构,BvGRAS可以分为九个亚科:LISCL,SHR,PAT1,SCR,SCL3、LAS、SCL4/7,HAM和DELLA。综合分析表明,BvGRAS基因中存在两对片段复制基因,表明基因复制不是BvGRAS家族成员的主要来源。调控网络分析显示,BvGRAS能够参与蛋白质相互作用的调控,材料运输,氧化还原平衡,离子稳态,渗透物质积累和植物形态结构影响甜菜对盐胁迫的耐受性。在盐胁迫下,BvGRAS及其靶基因呈上调表达趋势。其中,BvGRAS-15、BvGRAS-19、BvGRAS-20、BvGRAS-21、LOC104892636和LOC104893770可能是甜菜盐胁迫反应的关键基因。在这项研究中,分析了BvGRAS转录因子的结构特征和生物学功能,为进一步研究盐胁迫的分子机制和甜菜的分子育种提供了资料。
GAI-RGA-and-SCR (GRAS) transcription factors can regulate many biological processes such as plant growth and development and stress defense, but there are few related studies in sugar beet. Salt stress can seriously affect the yield and quality of sugar beet (Beta vulgaris). Therefore, this study used bioinformatics methods to identify GRAS transcription factors in sugar beet and analyzed their structural characteristics, evolutionary relationships, regulatory networks and salt stress response patterns. A total of 28 BvGRAS genes were identified in the whole genome of sugar beet, and the sequence composition was relatively conservative. According to the topology of the phylogenetic tree, BvGRAS can be divided into nine subfamilies: LISCL, SHR, PAT1, SCR, SCL3, LAS, SCL4/7, HAM and DELLA.
Synteny analysis showed that there were two pairs of fragment replication genes in the BvGRAS gene, indicating that gene replication was not the main source of BvGRAS family members. Regulatory network analysis showed that BvGRAS could participate in the regulation of protein interaction, material transport, redox balance, ion homeostasis, osmotic substance accumulation and plant morphological structure to affect the tolerance of sugar beet to salt stress. Under salt stress, BvGRAS and its target genes showed an up-regulated expression trend. Among them, BvGRAS-15, BvGRAS-19, BvGRAS-20, BvGRAS-21, LOC104892636 and LOC104893770 may be the key genes for sugar beet\'s salt stress response. In this study, the structural characteristics and biological functions of BvGRAS transcription factors were analyzed, which provided data for the further study of the molecular mechanisms of salt stress and molecular breeding of sugar beet.