PDF(Pubmed)
Abstract：
Adenosine kinase (ADK) is a key enzyme widely distributed in plants, playing an important role in maintaining cellular energy homeostasis and regulating plant growth, development, and responses to environmental stresses. However, research on ADK genes in cotton (Gossypium hirsutum), an economically significant crop, has been limited. This study identified 92 ADK genes from four cotton species (G. arboreum, G. raimondii, G. hirsutum, and G. barbadense) using HMMER and Local BLASTP methods and classified them into six groups. Chromosomal localization revealed a random distribution of ADK genes in G. hirsutum, with 13 genes located on the At subgenome and 14 genes on the Dt subgenome. Gene structure analysis showed consistency in exon-intron organization within subgroups, while conserved motif analysis identified subgroup-specific motifs, indicating functional diversity. Synteny and collinearity mapping analysis revealed that the primary expansion mechanisms of the ADK gene family in cotton are polyploidy and segmental duplication. Cis-regulatory elements in GhADK promoters were classified into light response, hormone response, developmental regulation, and stress response. We also analyzed the expression patterns of GhADK genes under a low temperature (4 °C) and drought conditions. Most GhADK genes responded to cold stress with different expression patterns, indicating their roles in rapid response and long-term cold adaptation. Under drought stress, expression patterns varied, with some genes showing sustained high expression levels. The qRT-PCR validation of transcriptomic data confirmed the stress-induced expression patterns of selected GhADK genes. Functional analysis through the VIGS silencing of GhADK25 demonstrated its importance in cold and drought stress responses, with silencing resulting in poor growth under stress, highlighting its significance in stress tolerance. This study provides a basis for further understanding the evolutionary relationships and functions of the cotton ADK gene family.
摘要：
腺苷激酶(adenosinekinase,ADK)是一种广泛分布于植物体内的关键酶,在维持细胞能量稳态和调节植物生长方面发挥着重要作用，发展,以及对环境压力的反应。然而,棉花ADK基因的研究,经济意义重大的作物，是有限的。这项研究鉴定了来自四个棉花物种的92个ADK基因（G。Arboreum,G.raimondii,G.hirsutum,和G.barbadense）使用HMMER和LocalBLASTP方法，并将其分为六组。染色体定位揭示了陆地棉中ADK基因的随机分布，有13个基因位于At亚基因组上，14个基因位于Dt亚基因组上。基因结构分析显示亚组内外显子-内含子组织的一致性，虽然保守的基序分析确定了亚组特异性基序，表明功能多样性。合成和共线性作图分析表明，棉花ADK基因家族的主要扩展机制是多倍体和节段复制。GhADK启动子中的顺式调控元件被分类为光响应，激素反应，发育调节，和应激反应。我们还分析了GhADK基因在低温（4°C）和干旱条件下的表达模式。大多数GhADK基因对寒冷胁迫的反应具有不同的表达模式，表明它们在快速反应和长期冷适应中的作用。在干旱胁迫下，表达模式各不相同，一些基因显示出持续的高表达水平。转录组数据的qRT-PCR验证证实了所选GhADK基因的应激诱导表达模式。通过GhADK25的VIGS沉默的功能分析证明了其在寒冷和干旱胁迫反应中的重要性。沉默导致压力下生长不良，强调其在抗逆性中的意义。本研究为进一步了解棉花ADK基因家族的进化关系和功能提供了基础。
