目的:头颅皮炎或游泳者瘙痒是由动物血吸虫穿透头颅引起的过敏性皮肤反应。它被认为是全球发现的人畜共患的水性皮肤疾病。在血吸虫吸虫中,禽类血吸虫是最负责的子囊皮炎。关于在泰国引起皮炎的尾c的发生知之甚少。因此,本研究的目的是通过形态学和分子学方法初步调查白药地区当地的lymnaeidae蜗牛中幼虫血吸虫感染的存在。
方法:总体500根(矢车菊)rubiginosa(米其林,1831年)是从PhayaoSanKwan村Phayao湖附近的淡水水库收集的,泰国,2020年10月至12月。通过子囊脱落技术,然后进行形态学和分子鉴定,检查了蜗牛的禽血吸虫感染。
结果:从6只感染的蜗牛(1.2%)中只观察到一种类型的多囊尾蚴。我们的分子分析表明,新兴的尾c与Trichobilharzia物种的28S核糖体RNA基因(28SrDNA)或细胞色素氧化酶C亚基1基因(cox1或COI)序列最相似。此外,两个基因座的系统发育树分析显示出相似的结果;新兴的尾c始终与Trichobilharziaregenti聚集在一起。
结论:我们的结果清楚地证实,检测到的发囊尾蚴属于毛虫属,并且与雷氏毛虫具有最高的同源性。这项研究提供了有关局部lymnaeidae蜗牛中引起尾c感染的皮炎发生的重要数据,鼓励有效管理,以及这种人畜共患传染病的控制措施。
OBJECTIVE: Cercarial dermatitis or swimmer\'s itch is an allergic skin reaction caused by penetrating cercaria of animal blood flukes. It is considered as a zoonotic water-borne skin condition that is found globally. Among the schistosomatid trematodes, avian schistosomes are the most responsible for cercarial dermatitis. Very little is known regarding the occurrence of dermatitis-causing cercariae in Thailand. Therefore, the objective of this study was to preliminarily investigate the presence of larval blood fluke infection among local lymnaeidae snails in Phayao by the incorporation of morphological and molecular methods.
METHODS: Overall 500 Radix (Lymnaea) rubiginosa (Michelin, 1831) were collected from freshwater reservoirs near Phayao Lake in San Kwan village in Phayao, Thailand, from October to December 2020. The snails were examined for avian blood fluke infection by the cercarial shedding technique followed by morphological and molecular characterization.
RESULTS: Only one type of furcocercous cercaria was observed to emerge from six infected snails (1.2%). Our molecular analyses demonstrated that the emerging cercariae showed most similarity to either the 28S ribosomal RNA gene (28S rDNA) or cytochrome oxidase C subunit 1 gene (cox1 or COI) sequences to those of Trichobilharzia species. In addition, phylogenetic tree analyses of both loci revealed similar results; the emerging cercariae were consistently clustered together with Trichobilharzia regenti.
CONCLUSIONS: Our results clearly confirmed that the detected furcocercous cercariae belonged to the genus Trichobilharzia and displayed the highest homology to T. regenti. This study provides important data on the occurrence of dermatitis causing cercariae infection among local lymnaeidae snails, encouraging effective management, and control measures for this zoonotic infectious disease.