假镰刀菌引起的镰刀菌冠腐病(FCR)对中国黄淮海地区的小麦生产构成了重大威胁。然而,假赤霉病的致病机制尚不清楚。Zn2Cys6转录因子,这是真菌独有的,在调节真菌发育中起关键作用,耐药性,致病性,和次生代谢。在这项研究中,我们介绍了Zn2Cys6转录因子F的功能表征。指定为Fp487。在F.seudograminearum,通过基因敲除和表型分析显示Fp487是菌丝体生长所必需的。与野生型CF14047相比,ΔFp487突变体的生长速率略有降低,但分生孢子发生显著降低,致病性和3-乙酰基-脱氧雪腐镰刀菌烯醇(3AcDON)的产生。此外,该突变体对氧化和细胞膜应激表现出更高的敏感性。此外,我们从Fp487基因体外合成dsRNA,通过喷雾诱导的基因沉默(SIGS),导致假赤霉病的生长速率及其在大麦叶片上的毒力降低。值得注意的是,本研究首次通过工程化大肠杆菌菌株HT115(DE3),并利用SIGS技术评估dsRNA对假赤霉病的毒力作用,从假赤霉病中诱导表达丰富的dsRNA。总之,我们的发现揭示了Fp487在调节致病性中的关键作用,应激反应,DON生产,和假赤霉病的分生孢子发生。此外,Fp487是FCR控制的潜在的基于RNAi的靶标。
Fusarium crown rot (FCR) caused by Fusarium pseudograminearum poses a significant threat to wheat production in the Huang-Huai-Hai region of China. However, the pathogenic mechanism of F. pseudograminearum is still poorly understood. Zn2Cys6 transcription factors, which are exclusive to fungi, play pivotal roles in regulating fungal development, drug resistance, pathogenicity, and secondary metabolism. In this study, we present the functional characterization of a Zn2Cys6 transcription factor F. pseudograminearum, designated Fp487. In F. pseudograminearum, Fp487 is shown to be required for mycelial growth through gene knockout and phenotypic analyses. Compared with wild-type CF14047, the ∆Fp487 mutant displayed a slight reduction in growth rate but a significant decrease in conidiogenesis, pathogenicity and 3-acetyl-deoxynivalenol (3AcDON) production. Moreover, the mutant exhibited heightened sensitivity to oxidative and cytomembrane stress. Furthermore, we synthesized dsRNA from the Fp487 gene in vitro, resulting in a reduction in the growth rate of F. pseudograminearum and its virulence on barley leaves through spray-induced gene silencing (SIGS). Notably, this study makes the first instance of inducing the expression of abundant dsRNA from F. pseudograminearum by engineering the Escherichia coli strain HT115 (DE3) and utilizing the SIGS technique to evaluate the virulence effect of dsRNA on F. pseudograminearum. In conclusion, our findings revealed the crucial role of Fp487 in regulating pathogenicity, stress responses, DON production, and conidiogenesis in F. pseudograminearum. Furthermore, Fp487 is a potential RNAi-based target for FCR control.