Abstract：
Sclerotinia disease is one of the most devastating fungal diseases worldwide, as it reduces the yields of many economically important crops. Pathogen-secreted effectors play crucial roles in infection processes. However, key effectors of Ciboria shiraiana, the pathogen primarily responsible for sclerotinia disease in mulberry (Morus spp.), remain poorly understood. In this study, we identified and functionally characterized the effector Cs02526 in C. shiraiana and found that Cs02526 could induce cell deathin a variety of plants. Moreover, Cs02526-induced cell death was mediated by the central immune regulator BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1 (BAK1), dependent on a 67-amino acid fragment. Notably, Cs02526 homologues were widely distributed in hemibiotrophic and necrotrophic phytopathogenic fungi, but the homologues failed to induce cell death in plants. Pre-treatment of plants with recombinant Cs02526 protein enhanced resistance against both C. shiraiana and Sclerotinia sclerotiorum. Furthermore, the pathogenicity of C. shiraiana was diminished upon spraying plants with synthetic dsRNA-Cs02526. In conclusion, our findings highlight the cell death-inducing effector Cs02526 as a potential target for future biological control strategies against plant diseases.
摘要：
菌核病是世界范围内最具破坏性的真菌疾病之一，因为它降低了许多经济上重要的作物的产量。病原体分泌的效应子在感染过程中起着至关重要的作用。然而,Shiraiana的关键影响因素，主要负责桑树菌核病的病原体（桑属物种。),仍然知之甚少。在这项研究中,我们鉴定并功能表征了Shiraiana中的效应子Cs02526，发现Cs02526可以诱导多种植物的细胞死亡。此外，Cs02526诱导的细胞死亡是由中枢免疫调节因子胆碱酯酶不敏感1相关受体激酶1（BAK1）介导的，依赖于67个氨基酸的片段。值得注意的是,Cs02526同源物广泛分布于半营养和坏死植物病原真菌中，但是同源物未能诱导植物细胞死亡。用重组Cs02526蛋白对植物进行预处理增强了对木薯和菌核病的抗性。此外，在用合成的dsRNA-Cs02526喷洒植物时,Shiraiana的致病性降低。总之,我们的研究结果强调了诱导细胞死亡的效应子Cs02526是未来植物病害生物防治策略的潜在靶点.
