Laccaria属(Hydnangiaceae,Agaricales)作为外生菌根真菌在森林生态系统中起着重要作用,通过与许多类型的树木共生促进营养循环。尽管了解了Laccaria的多样性和分布模式,以及它与寄主植物的联系,是建立平衡的植物多样性和进行有效的森林管理的基础,以前的研究在准确调查方面并不有效,因为他们严重依赖单独的标本收集。为了研究Laccaria物种的真实多样性和分布格局并确定其寄主类型,我们使用了四种不同的方法:基于样本的分析,开放数据库搜索(ODS),NGS分析,和物种特异性PCR(SSP)。因此,在韩国已确认了14种Laccaria。关于基于样本的分析和SSP的物种分布模式的结果不同。然而,当两者融合时,确定了每种Laccaria物种的确切分布模式。此外,SSP显示,许多Laccaria物种具有广泛的宿主类型。这项研究表明,使用这四种不同的方法有助于确定多样性,分布,和ECM真菌的宿主。此外,Laccaria获得的结果将作为基线,以帮助了解ECM真菌在应对气候变化的森林管理中的作用。
The genus Laccaria (Hydnangiaceae, Agaricales) plays an important role in forest ecosystems as an ectomycorrhizal fungus, contributing to nutrient cycles through symbiosis with many types of trees. Though understanding Laccaria diversity and distribution patterns, as well as its association with host plants, is fundamental to constructing a balanced plant diversity and conducting effective forest management, previous studies have not been effective in accurately investigating, as they relied heavily on specimen collection alone. To investigate the true diversity and distribution pattern of Laccaria species and determine their host types, we used four different approaches: specimen-based analysis, open database search (ODS), NGS analysis, and species-specific PCR (SSP). As a result, 14 Laccaria species have been confirmed in Korea. Results regarding the species distribution pattern were different between specimen-based analysis and SSP. However, when both were integrated, the exact distribution pattern of each Laccaria species was determined. In addition, the SSP revealed that many Laccaria species have a wide range of host types. This study shows that using these four different approaches is useful in determining the diversity, distribution, and host of ECM fungi. Furthermore, results obtained for Laccaria will serve as a baseline to help understand the role of ECM fungi in forest management in response to climate change.