{Reference Type}: Journal Article
{Title}: Recombinase Polymerase Amplification assay for detection of the British root-knot nematode, Meloidogyne artiellia.
{Author}: Subbotin SA;Palomares-Rius JE;Castillo P;
{Journal}: J Nematol
{Volume}: 56
{Issue}: 1
{Year}: 2024 Mar
{Factor}: 1.481
{DOI}: 10.2478/jofnem-2024-0023
{Abstract}: Recombinase polymerase amplification (RPA) is an isothermal in vitro nucleic acid amplification technique that has been adopted for simple, robust, rapid, reliable diagnostics of nematodes. In this study, the real-time RPA assay and RPA assay combined with lateral flow dipsticks (LF-RPA) have been developed targeting the ITS rRNA gene of the British root-knot nematode, Meloidogyne artiellia. The assay provided specific and rapid detection of this root-knot nematode species from crude nematode extracts without a DNA extraction step with a sensitivity of 0.125 second-stage juvenile (J2) specimen per a reaction tube for real-time RPA during 11 min and a sensitivity of 0.5 J2 specimens per a reaction tube for LF-RPA during 25 min. The RPA assays were validated with a wide range of non-target root-knot nematodes. The LF-RPA assay has great potential for nematode diagnostics in the laboratory having minimal available equipment.