%0 Journal Article
%T Recombinase Polymerase Amplification assay for detection of the British root-knot nematode, Meloidogyne artiellia.
%A Subbotin SA
%A Palomares-Rius JE
%A Castillo P
%J J Nematol
%V 56
%N 1
%D 2024 Mar
%M 38975564
%F 1.481
%R 10.2478/jofnem-2024-0023
%X Recombinase polymerase amplification (RPA) is an isothermal in vitro nucleic acid amplification technique that has been adopted for simple, robust, rapid, reliable diagnostics of nematodes. In this study, the real-time RPA assay and RPA assay combined with lateral flow dipsticks (LF-RPA) have been developed targeting the ITS rRNA gene of the British root-knot nematode, Meloidogyne artiellia. The assay provided specific and rapid detection of this root-knot nematode species from crude nematode extracts without a DNA extraction step with a sensitivity of 0.125 second-stage juvenile (J2) specimen per a reaction tube for real-time RPA during 11 min and a sensitivity of 0.5 J2 specimens per a reaction tube for LF-RPA during 25 min. The RPA assays were validated with a wide range of non-target root-knot nematodes. The LF-RPA assay has great potential for nematode diagnostics in the laboratory having minimal available equipment.