已经研究了在海洋大型藻类中发现的内生细菌的潜在抗菌活性,因此,它们可以作为控制病原菌的生物活性化合物的有价值的来源,酵母,和真菌。从Caulerpasp中分离出藻类内生细菌。,Ulvasp.,Ahnfeltiopsissp.,和来自Yacila和Cangrejo海滩的Chondracantuschamissoi(Piura,秘鲁)。使用交叉培养评估了针对病原菌的抗菌试验,过板,和挥发性有机化合物测试。之后,测定所选粗提物的最低抑菌浓度(MIC)和最低杀菌浓度(MBC),还有ITS分子分析,抗真菌活性,和iturin的PCR,芬霉素,并对表现出更好活性的细菌菌株进行了表面活性素基因。从藻类中分离出46株藻类内生细菌。10株抑制革兰氏阳性病原菌(粪肠球菌,表皮葡萄球菌,金黄色葡萄球菌,和单核细胞增生李斯特菌),和12抑制革兰氏阴性菌(大肠杆菌和沙门氏菌)。具有较好活性的细菌属于芽孢杆菌属。,Kluyvera抗坏血酸,成团的泛菌,十碳球,和肠杆菌。,其中只有四个对白色念珠菌具有抗真菌活性,C.热带,Colletotrichiumsp.,镰刀菌sp.,尖孢镰刀菌,和Alternariasp.此外,K.抗坏血酸YAFE21和芽孢杆菌。YCFE4表现出iturin和fengycin基因。结果表明,本研究发现的藻类内生细菌,特别是K.抗坏血酸YAFE21,芽孢杆菌属。YCFR6,十八烷基乳杆菌CUFE2,芽孢杆菌属。YUFE8,肠杆菌。YAFL1和成团假单胞菌YAFL6可以作为抗微生物化合物的潜在生产者进行研究,因为它们对各种微生物具有广泛的活性。
Endophytic bacteria found in marine macroalgae have been studied for their potential antimicrobial activity, consequently, they could serve as a valuable source of bioactive compounds to control pathogenic bacteria, yeasts, and fungi. Algae endophytic bacteria were isolated from Caulerpa sp., Ulva sp., Ahnfeltiopsis sp., and Chondracantus chamissoi from Yacila and Cangrejo Beaches (Piura, Peru). Antimicrobial assays against pathogenic bacteria were evaluated using cross-culture, over-plate, and volatile organic compound tests. Afterward, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of selected crude extracts were determined, also ITS molecular analysis, antifungal activity, and PCR of iturin, fengycin, and surfactin genes were performed for bacteria strains exhibiting better activity. Forty-six algae endophytic bacteria were isolated from algae. Ten strains inhibited gram-positive pathogenic bacteria (Enterococcus faecalis, Staphylococcus epidermidis, S. aureus, and Listeria monocytogenes), and 12 inhibited gram-negative bacteria (Escherichia coli and Salmonella enteric sv typhimurium). Bacteria with better activity belong to Bacillus sp., Kluyvera ascorbata, Pantoea agglomerans, Leclercia adecarboxylata, and Enterobacter sp., which only four showed antifungal activities against Candida albicans, C. tropicalis, Colletotrichium sp., Fusarium sp., Fusarium oxysporum, and Alternaria sp. Furthermore, K. ascorbata YAFE21 and Bacillus sp. YCFE4 exhibited iturin and fengycin genes. The results indicate that the algae endophytic bacteria found in this study, particularly K. ascorbata YAFE21, Bacillus sp. YCFR6, L. adecarboxylata CUFE2, Bacillus sp. YUFE8, Enterobacter sp. YAFL1, and P. agglomerans YAFL6, could be investigated as potential producers of antimicrobial compounds due to their broad activity against various microorganisms.