溃疡性结肠炎(UC)是一种常见的炎症性疾病,出现在结肠和直肠,全球患病率上升,严重影响患者的身心健康。UC治疗仍然存在重大挑战,强调需要安全有效的长期治疗方法。被誉为有希望的物理治疗,旋转磁场(RMF)证明了安全性,稳定性,可管理性,和效率。本研究探讨了RMF在减轻DSS诱导的小鼠UC中的潜力,评估疾病活动指数(DAI)和病理改变,如每日体重,粪便潜血,结肠长度,和形态变化。此外,已经检测到几个指标,包括促炎细胞因子的血清浓度(IL6,IL-17A,TNF-α,IFN-γ)和抗炎细胞因子(TGF-β,IL-4,IL-10),脾脏CD3+的比率,CD4+,和CD8+T细胞,结肠细胞凋亡率,结肠炎症和紧密连接相关蛋白的表达。结果表明,RMF对降低肠道通透性有有益作用,紧密连接的恢复,并通过减轻DSS诱导的UC模型小鼠结肠中的炎症功能障碍来减轻线粒体呼吸复合物(MRC)。总之,这项研究表明,RMF减弱结肠炎症,增强结肠紧密连接,并通过调节UC小鼠促炎和抗炎细胞因子的平衡来减轻MRC损伤,提示RMF在UC临床治疗中的潜在应用。
Ulcerative colitis (UC) is a prevalent inflammatory disorder that emerges in the colon and rectum, exhibiting a rising global prevalence and seriously impacting the physical and mental health of patients. Significant challenges remain in UC treatment, highlighting the need for safe and effective long-term therapeutic approaches. Heralded as a promising physical treatment, the rotating magnetic field (RMF) demonstrates safety, stability, manageability, and efficiency. This study delves into RMF\'s potential in mitigating DSS-induced UC in mice, assessing disease activity indices (DAI) and pathological alterations such as daily body weight, fecal occult blood, colon length, and morphological changes. Besides, several indexes have been detected, including serum concentrations of pro-inflammatory cytokines (IL6, IL-17A, TNF-α, IFN-γ) and anti-inflammatory cytokines (TGF-β, IL-4, IL-10), the ratio of splenic CD3+, CD4+, and CD8+ T cells, the rate of apoptotic colonic cells, the expression of colonic inflammatory and tight junction-associated proteins. The results showed that RMF had beneficial effects on the decrease of intestinal permeability, the restoration of tight junctions, and the mitigation of mitochondrial respiratory complexes (MRCs) by attenuating inflammatory dysfunction in colons of DSS-induced UC model of mice. In conclusion, this study demonstrates that RMF attenuates colonic inflammation, enhances colonic tight junction, and alleviates MRCs impairment by regulating the equilibrium of pro-inflammatory and anti-inflammatory cytokines in UC mice, suggesting the potential application of RMF in the clinical treatment of UC.