在生物学研究中对旋转磁场(RMF)的兴趣日益浓厚的背景下,关于RMF长期安全性的科学证据仍然存在显著差距。因此,这项研究旨在调查长期暴露于0.2T的安全性,在小鼠中经过10个月的4HzRMF。将两个月大的雌性C57BL/6小鼠随机分配到RMF组(暴露于0.2T,4Hz真实RMF)或SHAM组(暴露于0T,4Hz假RMF)。在整个实验过程中,记录了小鼠每周的体重,他们的行为特征是通过野外试验进行评估的。在最后一个月,对小鼠的整体健康状况进行了全面评估,包括血液参数的分析,主要器官的组织形态学检查,使用X射线和显微CT成像进行骨骼评估。通过免疫芯片分析和代谢组学评估小鼠免疫系统和脂质代谢。值得注意的是,没有观察到RMF暴露的明显不良反应.鼠体重,运动行为,器官组织形态学,骨骼健康不受RMF影响。血液分析显示,SHAM和RMF组之间的激素和脂质水平发生了细微变化,然而这些差异没有达到统计学意义.此外,RMF导致血清白细胞介素-28(IL-28)水平升高,尽管在正常范围内,和血清脂质代谢物的适度变化。最后,暴露于0.2T的小鼠,4HzRMF持续10个月没有显示出明显的慢性毒性迹象,表明其作为物理治疗的潜在临床应用。
Amidst the burgeoning interest in rotating magnetic fields (RMF) within biological research, there remains a notable gap in the scientific evidence concerning the long-term safety of RMF. Thus, this
study aimed to investigate the safety of protracted exposure to a 0.2 T, 4 Hz RMF over 10 months in mice. Two-month-old female C57BL/6 mice were randomly allocated to either the RMF group (exposed to 0.2 T, 4 Hz real RMF) or the SHAM group (exposed to 0 T, 4 Hz sham RMF). Throughout the experiment, the murine weekly body weights were recorded, and their behavioral traits were assessed via open field tests. In the final month, a comprehensive evaluation of the murine overall health was conducted, encompassing analyses of blood parameters, histomorphological examination of major organs, and skeletal assessments using X-ray and micro-CT imaging. The murine immune system and lipid metabolism were evaluated through immunochip analysis and metabolomics. Notably, no discernible adverse effects with RMF exposure were observed. Murine body weight, locomotor behavior, organ histomorphology, and skeletal health remained unaffected by RMF. Blood analysis revealed subtle changes in hormone and lipid levels between the SHAM and RMF groups, yet these differences did not reach statistical significance. Moreover, RMF led to elevated serum interleukin-28 (IL-28) levels, albeit within the normal range, and modest alterations in serum lipid metabolites. Conclusively, mice exposed to the 0.2 T, 4 Hz RMF for 10 months displayed no significant signs of chronic toxicity, indicating its potential clinical application as a physical therapy.