Abstract：
With the substantial increase in the overuse of glucocorticoids (GCs) in clinical medicine, the prevalence of glucocorticoid-induced osteonecrosis of the femoral head (GC-ONFH) continues to rise in recent years. However, the optimal treatment for GC-ONFH remains elusive. Rotating magnetic field (RMF), considered as a non-invasive, safe and effective approach, has been proved to have multiple beneficial biological effects including improving bone diseases. To verify the effects of RMF on GC-ONFH, a lipopolysaccharide (LPS) and methylprednisolone (MPS)-induced invivo rat model, and an MPS-induced invitro cell model have been employed. The results demonstrate that RMF alleviated bone mineral loss and femoral head collapse in GC-ONFH rats. Meanwhile, RMF reduced serum lipid levels, attenuated cystic lesions, raised the expression of anti-apoptotic proteins and osteoprotegerin (OPG), while suppressed the expression of pro-apoptotic proteins and nuclear factor receptor activator-κB (RANK) in GC-ONFH rats. Besides, RMF also facilitated the generation of ALP, attenuated apoptosis and inhibits the expression of pro-apoptotic proteins, facilitated the expression of OPG, and inhibited the expression of RANK in MPS-stimulated MC3T3-E1 cells. Thus, this study indicates that RMF can improve GC-ONFH in rat and cell models, suggesting that RMF have the potential in the treatment of clinical GC-ONFH.
摘要：
随着糖皮质激素(GCs)在临床医学中过度使用的大幅增加,近年来,糖皮质激素性股骨头坏死(GC-ONFH)的患病率持续上升.然而,GC-ONFH的最佳治疗方法仍然难以捉摸。旋转磁场(RMF),被认为是非侵入性的，安全有效的方法，已被证明具有多种有益的生物学效应，包括改善骨骼疾病。为了验证RMF对GC-ONFH的影响，脂多糖（LPS）和甲基强的松龙（MPS）诱导的体内大鼠模型，并采用了MPS诱导的体外细胞模型。结果表明，RMF减轻了GC-ONFH大鼠的骨矿物质丢失和股骨头塌陷。同时,RMF降低血清脂质水平，减弱的囊性病变，提高抗凋亡蛋白和骨保护素（OPG）的表达，同时抑制GC-ONFH大鼠促凋亡蛋白和核因子受体激活物-κB（RANK）的表达。此外,RMF还促进了ALP的产生，减弱凋亡并抑制促凋亡蛋白的表达，促进OPG的表达，并抑制MPS刺激的MC3T3-E1细胞中RANK的表达。因此，这项研究表明，RMF可以改善大鼠和细胞模型的GC-ONFH，提示RMF具有治疗临床GC-ONFH的潜力。
