■日粮中的粗蛋白水平会影响发酵参数,微生物,反刍动物瘤胃中的代谢产物.研究补充日粮中粗蛋白水平对微生物群落及代谢产物的影响对提高动物生长性能具有重要意义。目前,补充日粮粗蛋白水平对瘤胃发酵参数的影响,微生物群落,泽西牦牛(JY)的代谢产物尚不清楚。
■本实验的目的是研究JY日粮中合适的粗蛋白水平。通过粗蛋白质水平为15.16和17.90%的补充日粮测定瘤胃发酵指标(挥发性脂肪酸和pH),分别,利用非靶标代谢组学和宏基因组测序技术对JYs的微生物群落和代谢产物进行分析,瘤胃发酵参数的变化,微生物菌群,研究了三组中的代谢物及其相互作用。
■补充日粮中的粗蛋白水平对pH有显著影响,戊酸,乙酸与丙酸的比例(p<0.05)。蛋白质水平对门水平的优势微生物区系无显著影响(p>0.05),三组均为拟杆菌和厚壁菌。代谢产物分析结果表明,补充日粮粗蛋白水平显著影响胆汁分泌和苯乙烯降解等代谢途径(p<0.05),LP组和HP组之间有不同的代谢产物,这些不同的代谢产物在一定程度上与优势微生物有关。总而言之,在这个实验中,研究了日粮中粗蛋白水平对JY瘤胃微生物和代谢产物的影响及其相互关系,为今后制定更加科学合理的补充膳食提供了理论依据。
UNASSIGNED: The crude protein level in the diet will affect the fermentation parameters, microflora, and metabolites in the rumen of ruminants. It is of great significance to study the effect of crude protein levels in supplementary diet on microbial community and metabolites for improving animal growth performance. At present, the effects of crude protein level in supplementary diet on rumen fermentation parameters, microbial community, and metabolites of Jersey-Yak (JY) are still unclear.
UNASSIGNED: The purpose of this experiment was to study the appropriate crude protein level in the diet of JY. The rumen fermentation indexes (volatile fatty acids and pH) were determined by supplementary diets with crude protein levels of 15.16 and 17.90%, respectively, and the microbial community and metabolites of JYs were analyzed by non-target metabonomics and metagenome sequencing technology, and the changes of rumen fermentation parameters, microbial flora, and metabolites in the three groups and their interactions were studied.
UNASSIGNED: The crude protein level in the supplementary diet had significant effects on pH, valeric acid, and the ratio of acetic acid to propionic acid (p < 0.05). The protein level had no significant effect on the dominant microflora at the phylum level (p > 0.05), and all three groups were Bacteroides and Firmicutes. The results of metabolite analysis showed that the crude protein level of supplementary diet significantly affected the metabolic pathways such as Bile secretion and styrene degradation (p < 0.05), and there were different metabolites between the LP group and HP group, and these different metabolites were related to the dominant microbial to some extent. To sum up, in this experiment, the effects of crude protein level in supplementary diet on rumen microorganisms and metabolites of JY and their relationship were studied, which provided the theoretical basis for formulating a more scientific and reasonable supplementary diet in the future.