PDF(Pubmed)
Abstract：
Head and neck squamous cell carcinomas (HNSCCs) are one of the most frequently detected cancers in the world; not all mechanisms related to the expression of keratin in this type of cancer are known. The aim of this study was to evaluate type II cytokeratins (KRT): KRT6A, KRT6B, and KRT6C protein concentrations in 54 tumor and margin samples of head and neck squamous cell carcinoma (HNSCC). Moreover, we examined a possible association between protein concentration and the clinical and demographic variables. Protein concentrations were measured using enzyme-linked immunosorbent assay (ELISA). Significantly higher KRT6A protein concentration was found in HNSCC samples compared to surgical margins. An inverse relationship was observed for KRT6B and KRT6C proteins. We showed an association between the KRT6C protein level and clinical parameters T and N in tumor and margin samples. When analyzing the effect of smoking and drinking on KRT6A, KRT6B, and KRT6C levels, we demonstrated a statistically significant difference between regular or occasional tobacco and alcohol habits and patients who do not have any tobacco and alcohol habits in tumor and margin samples. Moreover, we found an association between KRT6B and KRT6C concentration and proliferative index Ki-67 and HPV status in tumor samples. Our results showed that concentrations of KRT6s were different in the tumor and the margin samples and varied in relation to clinical and demographic parameters. We add information to the current knowledge about the role of KRT6s isoforms in HNSCC. We speculate that variations in the studied isoforms of the KRT6 protein could be due to the presence and development of the tumor and its microenvironment. It is important to note that the analyses were performed in tumor and surgical margins and can provide more accurate information on the function in normal and cancer cells and regulation in response to various factors.
摘要：
头颈部鳞状细胞癌（HNSCC）是世界上最常见的癌症之一；并非所有与此类癌症中角蛋白表达相关的机制都是已知的。这项研究的目的是评估II型细胞角蛋白（KRT）：KRT6A，KRT6B,54例头颈部鳞状细胞癌（HNSCC）肿瘤和边缘样本中的KRT6C蛋白浓度。此外，我们研究了蛋白质浓度与临床和人口统计学变量之间的可能关联.使用酶联免疫吸附测定(ELISA)测量蛋白质浓度。与手术边缘相比,在HNSCC样品中发现显著更高的KRT6A蛋白浓度。观察到KRT6B和KRT6C蛋白的反比关系。我们显示了肿瘤和边缘样本中KRT6C蛋白水平与临床参数T和N之间的关联。在分析吸烟和饮酒对KRT6A的影响时，KRT6B,和KRT6C电平，我们在肿瘤和边缘样本中证明了有规律或偶尔吸烟和饮酒习惯的患者与没有任何吸烟和饮酒习惯的患者之间存在统计学上的显著差异.此外，我们发现肿瘤样本中KRT6B和KRT6C浓度与增殖指数Ki-67和HPV状态之间存在相关性.我们的结果表明，肿瘤和边缘样本中KRT6的浓度不同，并且与临床和人口统计学参数有关。我们将有关KRT6s同工型在HNSCC中的作用的信息添加到当前知识中。我们推测，所研究的KRT6蛋白同种型的变化可能是由于肿瘤及其微环境的存在和发展所致。重要的是要注意，分析是在肿瘤和手术边缘进行的，可以提供有关正常和癌细胞功能以及响应于各种因素的调节的更准确信息。
