生物活性天然产物是药物开发的重要组成部分。糖尿病受试者中的蛋白质糖基化导致糖尿病并发症如肾病和神经病变。我们研究了石榴己烷提取物(PHE)作为抗氧化剂的影响,抗炎,糖尿病大鼠的抗糖基化作用。PHE的气相色谱/质谱(GC/MS)分析显示存在间苯二酚,儿茶酚,tau-cadinol,甲氨酚,scopoletin,植物醇,和苯酚,3-十五烷基是与生物活性有关的最具活性成分。获得的结果表明,与未治疗的糖尿病大鼠相比,PHE增加了血清醛糖还原酶和总抗氧化活性(p<0.001)。此外,PHE通过增强来发挥抗氧化剂,过氧化氢酶和SOD(p<0.001)和降低MDA(p<0.001),通过抑制1β(IL-1β)的产生进行抗炎,肿瘤坏死因子(TNF-α)(p<0.001),与未治疗组相比,糖尿病大鼠的肾病和AGEs(p<0.001)。结论是,石榴有望开发一种功能性生物分子,用于治疗和预防糖尿病肾病等并发症。需要更多的研究来研究这些分子的分子作用。
Bioactive natural products are essential components for drug development. Protein glycation in diabetic subjects leads to diabetic complications as nephropathy and neuropathy. We investigated the impact of pomegranate hexane extract (PHE) as antioxidant, anti-inflammatory, and antiglycation in diabetic rats. Gas chromatography/mass spectrum (GC/MS) analysis of PHE revealed presence of resorcinol, catechol, tau-cadinol, metacetamol, scopoletin, phytol, and phenol, 3-pentadecyl as the most active ingredients that related to biological activity. Results obtained showed that, PHE increased serum aldose reductase and total antioxidant activity compared with untreated diabetic rats (p < 0.001). In addition, PHE exert antioxidant by enhancing, catalase and SOD (p < 0.001) and decreased MDA (p < 0.001), anti-inflammatory by inhibition production of 1 β (IL-1β), tumor necrosis factor (TNF-α) (p < 0.001), and AGEs (p < 0.001) against nephropathy in diabetic rats compared with untreated group. It was concluded that, pomegranate is promising in development a functional biomolecule in treatment and protection against diabetic complications as nephropathy. More study required to investigate the molecular action of these molecules.