PDF(Pubmed)
Abstract：
Amidst the growing concern of antimicrobial resistance as a significant health challenge, research has emerged, focusing on elucidating the antimicrobial potential of polyphenol-rich extracts to reduce reliance on antibiotics. Previous studies explored the antifungal effects of extracts as potential alternatives to conventional therapeutic strategies. We aimed to assess the antibacterial and antifungal effects of standardised pomegranate extract (PE) and lemon extract (LE) using a range of Gram-negative and Gram-positive bacteria and two yeast species. Additionally, we assessed the antimicrobial activities of common antibiotics (Ciprofloxacin, Imipenem, Gentamicin, and Ceftazidime), either alone or in combination with extracts, against Staphylococcus aureus and Escherichia coli. PE displayed substantial antibacterial (primarily bactericidal) and antifungal effects against most pathogens, while LE exhibited antibacterial (mostly bacteriostatic) and antifungal properties to a lesser extent. When compared with antibiotics, PE showed a greater zone of inhibition (ZOI) than Ciprofloxacin and Ceftazidime (p < 0.01) and comparable ZOI to Gentamicin (p = 0.4) against Staphylococcus aureus. However, combinations of either PE or LE with antibiotics exhibited either neutral or antagonistic effects on antibiotic activity against Staphylococcus aureus and Escherichia coli. These findings contribute to the existing evidence regarding the antimicrobial effects of PE and LE. They add to the body of research suggesting that polyphenols exert both antagonistic and synergistic effects in antimicrobial activity. This highlights the importance of identifying optimal polyphenol concentrations that can enhance antibiotic activity and reduce antibiotic resistance. Further in vivo studies, starting with animal trials and progressing to human trials, may potentially lead to recommendation of these extracts for therapeutic use.
摘要：
在抗菌素耐药性作为重大健康挑战的日益关注中，研究已经出现，重点阐明富含多酚的提取物的抗菌潜力，以减少对抗生素的依赖。先前的研究探索了提取物作为常规治疗策略的潜在替代品的抗真菌作用。我们旨在使用一系列革兰氏阴性和革兰氏阳性细菌以及两种酵母菌种评估标准化石榴提取物（PE）和柠檬提取物（LE）的抗菌和抗真菌作用。此外,我们评估了常见抗生素(环丙沙星，亚胺培南,庆大霉素,和头孢他啶)，单独或与提取物组合，针对金黄色葡萄球菌和大肠杆菌。PE对大多数病原体表现出明显的抗菌（主要是杀菌）和抗真菌作用，而LE表现出较小的抗菌（主要是抑菌）和抗真菌特性。与抗生素相比，PE对金黄色葡萄球菌显示出比环丙沙星和头孢他啶更大的抑制区域(ZOI)(p<0.01)和与庆大霉素相当的ZOI(p=0.4)。然而,PE或LE与抗生素的组合对金黄色葡萄球菌和大肠杆菌的抗生素活性表现出中性或拮抗作用。这些发现有助于现有的关于PE和LE的抗微生物作用的证据。他们增加了研究的主体，表明多酚在抗微生物活性中既发挥拮抗作用又发挥协同作用。这突出了确定可以增强抗生素活性和降低抗生素抗性的最佳多酚浓度的重要性。进一步的体内研究，从动物试验开始，发展到人体试验，可能会导致推荐这些提取物用于治疗用途。
