背景:蒿叶(AC)叶因其在传统医学中的重要性而被广泛认可。尽管对这个植物进行了大量的研究,在突尼斯生长的叶子的化学成分和生物活性仍未得到充分研究。在这项对AC的研究中,采用连续提取方法(己烷,乙酸乙酯和甲醇)通过LC-MS分析研究其生物活性成分,和它们的抗氧化剂,抗菌,抗真菌药,和抗癌活动。
结果:数据分析显示AC提取物中不同的化合物谱。甲醇和乙酸乙酯提取物具有较高的多酚含量和抗氧化活性,而己烷表现出优越的植物甾醇提取。乙酸乙酯提取物对多重耐药金黄色葡萄球菌和铜绿假单胞菌显示出有效的抗菌活性。此外,所有展示的提取物,第一次,对黄曲霉和黑曲霉具有强大的抗真菌功效。细胞毒性试验显示甲醇和乙酸乙酯提取物对转移性乳腺癌和多发性骨髓瘤的显著影响,在我们的研究中第一次检查。此外,对多发性骨髓瘤细胞的进一步分析强调,乙酸乙酯提取物诱导凋亡和坏死细胞死亡,并导致S期细胞周期阻断,强调其治疗潜力。
结论:这项调查发现了突尼斯AC的新发现,特别是羽扇豆醇的鉴定,齐墩果酸,熊果酸,豆甾醇和β-谷甾醇。该研究揭示了AC提取物在治疗干预中的有希望的作用,并强调需要继续研究以充分发挥其在医学和药物开发中的潜力。
BACKGROUND: Artemisia campestris L. (AC) leaves are widely recognized for their importance in traditional medicine. Despite the considerable amount of research conducted on this plant overworld, the chemical composition and the biological activity of the leaves grown in Tunisia remains poorly investigated. In this study of AC, a successive extraction method was employed (hexane, ethyl acetate and methanol) to investigate its bioactive constituents by LC-MS analysis, and their antioxidant, antibacterial, antifungal, and anticancer activities.
RESULTS: Data analysis revealed diverse compound profiles in AC extracts. Methanolic and ethyl acetate extracts exhibited higher polyphenolic content and antioxidant activities, while Hexane showed superior
phytosterol extraction. Ethyl acetate extract displayed potent antibacterial activity against multi-resistant Staphylococcus aureus and Pseudomonas aeruginosa. Additionally, all extracts demonstrated, for the first time, robust antifungal efficacy against Aspergillus flavus and Aspergillus niger. Cytotoxicity assays revealed the significant impact of methanolic and ethyl acetate extracts on metastatic breast cancer and multiple myeloma, examined for the first time in our study. Moreover, further analysis on multiple myeloma cells highlighted that the ethyl acetate extract induced apoptotic and necrotic cell death and resulted in an S phase cell cycle blockage, underscoring its therapeutic potential.
CONCLUSIONS: This investigation uncovers novel findings in Tunisian AC, notably the identification of lupeol, oleanolic acid, ursolic acid, stigmasterol and β-sitosterol. The study sheds light on the promising role of AC extracts in therapeutic interventions and underscores the need for continued research to harness its full potential in medicine and pharmaceutical development.