Abstract：
Phytosterol (PS) is a steroid, and its bioavailability can be enhanced by interacting with protein in the C-24 hydroxyl group. The interaction between sterols and amino acid residues in proteins can be enhanced by enzymatic hydrolysis. Phytosterol and whey insulation hydrolysates (WPH1-4) fabricated by the Alcalase enzyme at different enzymatic hydrolysis times were selected as delivery systems to simulate sterol C-24 hydroxyl group interaction with protein. Increasing hydrolysis time can promote the production of β-Lg, which raises the ratio of β-turn in the secondary structure and promotes the formation of interaction between WPH and PS. The correlation coefficient between hydrogen bonds and encapsulation efficiency (EE) and bioaccessibility is 0.91 and 0.88 (P < 0.05), respectively, indicating that hydrogen bonds of two components significantly influenced the combination by concealing the hydrophobic amino acids and some residues, which improved PS EE and bioavailability by 3.03 and 2.84 times after PS was combined with the WPI hydrolysate. These findings are expected to enhance the absorption of PS and other macromolecules by protein enzymatic hydrolysis to broaden their applications for food.
摘要：
植物甾醇（PS）是一种类固醇，其生物利用度可以通过与C-24羟基中的蛋白质相互作用来提高。酶水解可以增强甾醇与蛋白质中氨基酸残基之间的相互作用。选择由Alcalase酶在不同的酶促水解时间制备的植物甾醇和乳清绝缘水解产物（WPH1-4）作为递送系统，以模拟甾醇C-24羟基与蛋白质的相互作用。增加水解时间可以促进β-Lg的产生，这提高了二级结构中β转角的比例，并促进了WPH和PS之间相互作用的形成。氢键与包封率(EE)和生物可及性的相关系数分别为0.91和0.88(P<0.05),分别,说明两种组分的氢键通过隐藏疏水性氨基酸和一些残基显著影响了组合，PS与WPI水解产物组合后，PSEE和生物利用度分别提高了3.03和2.84倍。这些发现有望通过蛋白酶水解增强PS和其他大分子的吸收，以扩大其在食品中的应用。
