目的:与2型糖尿病(T2DM)相关的高发病率和死亡率构成了重大的全球卫生挑战。需要开发副作用更少的更有效的抗糖尿病药物。本研究探讨维生素D3联合格列本脲对2型糖尿病大鼠的干预作用,阐明其通过NF-κB通路对胰岛β细胞的影响。
方法:将24只健康雄性SD大鼠随机分为4组:对照组模型组(MG),格列本脲(GG)组,和格列本脲+维生素D3组(GDG)。高脂高糖饮食和腹腔注射链脲佐菌素诱导T2DM模型后,GG组大鼠口服格列本脲(0.6mg/kg/天),而GDG组的患者在玉米油中同时接受格列本脲(0.6mg/kg/天)和维生素D3(500IU/kg/天),为期8周。测量生化指标,用苏木素和伊红染色观察胰腺组织和胰岛β细胞的组织病理学变化。胰腺核因子κB(NF-κB)的表达,胰岛β细胞,使用TUNEL方法和PCR评估炎性细胞因子。
结果:根据当前研究的数据,GDG组血浆生化指标呈显著正差异,以及β细胞的表达,NF-κBp65,TNF-α,IL-1β,INF-γ,和Fas,GG组和CG组比较(P<0.05)。
结论:结果提示维生素D通过抑制NF-κB信号通路改善胰岛β细胞功能,对T2DM具有有益作用。因此,建议补充维生素D,当与抗糖尿病药物一起使用时,可以更有效地预防和治疗T2DM。
OBJECTIVE: The high morbidity and mortality associated with type 2 diabetes mellitus (T2DM) pose a significant global health challenge, necessitating the development of more efficient anti-diabetic drugs with fewer side effects. This study investigated the intervention of vitamin D3 combined with glibenclamide in rats with T2DM to elucidate its effects on pancreatic β-cells through the NF-κB pathway.
METHODS: Twenty-four healthy male Sprague-Dawley (SD) rats were randomly assigned to four groups: the control group (CG), the model group (MG), the glibenclamide group (GG), and the glibenclamide + vitamin D3 group (GDG). After inducing the T2DM model using high-fat and high-sugar diet and intraperitoneal injection of streptozotocin, the rats in the GG group were administered glibenclamide orally (0.6 mg/kg/day), while those in the GDG group received both glibenclamide (0.6 mg/kg/day) and vitamin D3 (500 IU/kg/day) in corn oil for a duration of 8 weeks. Biochemical indices were measured, and histopathological changes in pancreatic tissue and islet β cells were observed using hematoxylin and eosin staining. The expression of pancreatic nuclear factor κB (NF-κB), islet β-cells, and inflammatory cytokines were assessed using the TUNEL method and PCR.
RESULTS: According to the data from this current study, the GDG group showed significant positive differences in plasma biochemical indices, as well as in the expression of β cells, NF-κB p65, TNF-α, IL-1β, INF-γ, and Fas, compared to the GG and CG groups (P < 0.05).
CONCLUSIONS: The results suggest that vitamin D has beneficial effects on T2DM by improving the functions of islet β cells through inhibition of the NF-κB signaling pathway. Therefore, it is suggested that vitamin D supplementation, when used alongside antidiabetic drugs, may more effectively prevent and treat T2DM.