PDF(Pubmed)
Abstract：
Antibiotic overuse in poultry feeds has disastrous implications; consequently, long-term alternatives must be developed. As a result, the current study aims to assess the impact of Aspergillus niger filtrate (ANF) high in organic acids grown on agro-industrial residue of faba bean (AIRFB) on quail diet, as well as their influence on bird productivity, digestion, carcass yield, blood chemistry, and intestinal microbiota. A total of 240 Japanese quails (aged 7 d) were used in this study, divided equally among 5 experimental groups with 48 quails each. Group 1 (G1) received a basal diet without any ANF, group 2 (G2) received a basal diet supplemented with 0.5 mL ANF/kg diet, group 3 (G3) received a basal diet supplemented with 1.0 mL ANF/kg diet, group 4 (G4) received a basal diet supplemented with 1.5 mL ANF/kg diet, and group 5 (G5) received a basal diet supplemented with 2 mL ANF/kg diet. The performance parameters were monitored at 1 to 3, 3 to 5, and 1 to 5 wk. Adding ANF increased body weight at 3 and 5 wk, as well as body weight gain at 1 to 3, 3 to 5, and 1 to 5 wk, compared to the control diet. The ANF fed quails had the highest feed conversion ratio compared to the control group. The addition of ANF to the quail diet had no effect on the weight of the carcass, gizzard, heart, liver, giblets, or dressing; however, it did lower triglycerides, low-density lipoprotein, and very low-density lipoprotein while increasing high-density lipoprotein levels. The quail groups that received ANF had enhanced immunological indices such as IgG, IgM, IgA, and lysozymes. It also increased the levels of superoxide dismutase and total antioxidant contents, as well as catalase, and digestive enzymes such as protease, amylase, and lipase. However, it lowered the blood MDA levels compared to control. It has been demonstrated that the total gut microbiota, Escherichia coli, total coliforms, and the population of Salmonella are all reduced in ANF-fed quails. Histological examination of ANF quails\' liver and intestinal sections revealed normal hepatic parenchyma, typical leaf-like intestinal villi, and comparatively short and frequently free lumina. In conclusion, Japanese quail showed improvements in performance, digestive enzymes, antioxidant indices, immunity, and capacity to reduce intestinal pathogenic bacteria after consuming diet supplemented with ANF.
摘要：
在家禽饲料中过度使用抗生素会带来灾难性的影响；因此，必须开发长期替代品。因此,本研究旨在评估黑曲霉滤液（ANF）中有机酸含量高的蚕豆（AIRFB）对鹌鹑饮食的影响，以及它们对鸟类生产力的影响，消化，car体产量，血液化学,和肠道微生物群。本研究共使用240只日本鹌鹑(7日龄)，平均分为5个实验组，每组48只鹌鹑。第1组（G1）接受无任何ANF的基础饮食，第2组（G2）接受补充0.5mLANF/kg饮食的基础饮食，第3组（G3）接受补充1.0mLANF/kg饮食的基础饮食，第4组（G4）接受补充1.5mLANF/kg饮食的基础饮食，第5组（G5）接受补充2mLANF/kg饮食的基础饮食。在1至3周、3至5周和1至5周监测性能参数。添加ANF在3周和5周时增加了体重，以及在1至3周、3至5周和1至5周体重增加，与对照饮食相比。与对照组相比，饲喂ANF的鹌鹑的饲料转化率最高。鹌鹑饮食中添加ANF对car体的重量没有影响，Gizzard,心,肝脏,内脏,或敷料；然而，它确实降低了甘油三酯，低密度脂蛋白，和极低密度脂蛋白，同时增加高密度脂蛋白水平。接受ANF的鹌鹑组的免疫学指标增强，例如IgG，IgM,IgA,和溶菌酶.它还增加了超氧化物歧化酶和总抗氧化剂含量的水平，以及过氧化氢酶，和消化酶，如蛋白酶，淀粉酶，和脂肪酶。然而,与对照组相比，它降低了血液MDA水平。已经证明，总的肠道微生物群，大肠杆菌,总大肠菌群，ANF喂养的鹌鹑中沙门氏菌的数量都减少了。ANF鹌鹑肝脏和肠道切片的组织学检查显示肝实质正常，典型的叶状肠绒毛,和相对短且经常自由的发光。总之,日本鹌鹑的表现有所改善，消化酶，抗氧化剂指数，豁免权,以及食用补充ANF的饮食后减少肠道致病菌的能力。
