背景:亚硫酸氢盐(HSO3-)用作漂白剂,抗氧化剂,抗菌,和生物系统中酶促反应的调节剂。然而,亚硫酸氢盐含量异常对健康有害。次氯酸(HOCl),作为生物活性小分子,对于维持生物体的正常生物学功能至关重要。其平衡的破坏可导致氧化应激和各种疾病。因此,监测HOCl和HSO3-在细胞和体内水平的波动对研究其生理和病理功能至关重要。
结果:这项研究利用噻吩香豆素-茚二酮结构构建了一种新型的NIR双功能比色荧光探针,以鉴定次氯酸盐(ClO-)和亚硫酸氢盐(HSO3-)。通过使用CSO-IO识别HSO3-和HOCl,产生了两种不同的产品,显示绿色和蓝色荧光,分别。该性质有效地允许同时双功能检测HSO3-(LOD:113nM)和HOCl(LOD:43nM)。
结论:在这项工作中,生物相容性分子CSO-IO已被有效设计用于检测活细胞和斑马鱼中的HOCl/HSO3-。因此,双功能荧光探针有可能作为分子工具在复杂的生物系统中同时检测HSO3-衍生化合物和HOCl。
BACKGROUND: Bisulfite (HSO3-) serves as a bleaching agent, antioxidant, antimicrobial, and regulator of enzymatic reactions in biosystem. However, abnormal levels of bisulfite can be detrimental to health. Hypochlorous acid (HOCl), which acts as bioactive small molecules, is crucial for maintaining normal biological functions in living organisms. Disruption of its equilibrium can lead to oxidative stress and various diseases. Therefore, it\'s essential to monitor the fluctuations of HOCl and HSO3- at cellular and in vivo levels to study their physiological and pathological functions.
RESULTS: This study constructed a novel NIR bifunctional colorimetric fluorescent probe using thienocoumarin-indanedione structures to identify hypochlorite (ClO-) and bisulfite (HSO3-). By using CSO-IO to recognize HSO3- and HOCl, two distinct products were generated, displaying green and blue fluorescence, respectively. This property effectively allows for the simultaneous dual-functional detection of HSO3- (LOD: 113 nM) and HOCl (LOD: 43 nM).
CONCLUSIONS: In this work, the biocompatible molecule CSO-IO has been effectively designed to detect HOCl/HSO3- in living cells and zebrafish. As a result, the dual-functional fluorescent probe has the potential to be utilized as a molecular tool to detect HSO3- derived compounds and HOCl simultaneously within the complex biological system.