PDF(Pubmed)
Abstract：
Photothermal therapy (PTT) for cancers guided by optical imaging has recently shown great potential for precise diagnosis and efficient therapy. The second near-infrared window (NIR-II, 1000-1700 nm) fluorescence imaging (FLI) is highly desirable owing to its good spatial and temporal resolution, deep tissue penetration, and negligible tissue toxicity. Organic small molecules are attractive as imaging and treatment agents in biomedical research because of their low toxicity, fast clearance rate, diverse structures, ease of modification, and excellent biocompatibility. Various organic small molecules have been investigated for biomedical applications. However, there are few reports on the use of croconaine dyes (CRs), especially NIR-II emission CRs. To our knowledge, there have been no prior reports of NIR-II emissive small organic photothermal agents (SOPTAs) based on CRs. Herein, we report a croconaine dye (CR-TPE-T)-based nanoparticle (CR NP) with absorption and fluorescence emission in the NIR-I and NIR-II windows, respectively. The CR NPs exhibited intense NIR absorption, outstanding photothermal properties, and good biological compatibility. In vivo studies showed that CR NPs not only achieved real-time, noninvasive NIR-II FLI of tumors, but also induced significant tumor ablation with laser irradiation guided by imaging, without apparent side effects, and promoted the formation of antitumor immune memory in a colorectal cancer model. In addition, the CR NPs displayed efficient inhibition of breast tumor growth, improved longevity of mice and triggered efficient systemic immune responses, which further inhibited tumor metastasis to the lungs. Our study demonstrates the great potential of CRs as therapeutic agents in the NIR-II region for cancer diagnosis.
摘要：
通过光学成像引导的癌症光热治疗（PTT）最近显示出精确诊断和有效治疗的巨大潜力。第二个近红外窗口（NIR-II，1000-1700nm）荧光成像（FLI）由于其良好的空间和时间分辨率而非常理想，深层组织穿透，和可忽略的组织毒性。有机小分子由于其低毒性而在生物医学研究中作为成像和治疗剂具有吸引力。快速清除率，不同的结构，易于修改，和优良的生物相容性。已经研究了各种有机小分子用于生物医学应用。然而,很少有关于使用croconaine染料（CRs）的报道，特别是NIR-II发射CRs。据我们所知,以前没有基于CRs的NIR-II发射性小型有机光热剂（SOPTAs）的报道。在这里，我们报告了一种基于croconaine染料（CR-TPE-T）的纳米颗粒（CRNP），在NIR-I和NIR-II窗口中具有吸收和荧光发射，分别。CRNP表现出强烈的近红外吸收，出色的光热性能，良好的生物相容性。体内研究表明，CRNP不仅实现了实时，肿瘤的非侵入性NIR-IIFLI，而且还通过成像引导激光照射诱导了显著的肿瘤消融，没有明显的副作用，并促进结直肠癌模型中抗肿瘤免疫记忆的形成。此外,CRNP显示有效抑制乳腺肿瘤生长，改善小鼠的寿命并引发有效的全身免疫反应，这进一步抑制了肿瘤向肺部的转移。我们的研究证明了CRs作为NIR-II区域癌症诊断的治疗剂的巨大潜力。
