通过高通量测序(HTS)对具有叶片斑驳症状的橄榄树病毒的研究揭示了一种新病毒的存在。确定了两个分离株的完整编码基因组序列,分别由16,516nt和16,489的单个RNA片段组成。基因组组织包含10个开放阅读框(ORF),从5'到3':ORF1a,ORF1b(RdRp),ORF2(p22),ORF3(p7),ORF4(HSP70h),ORF5(HSP90h),ORF6(CP),ORF7(p19),ORF8(p12),ORF9(p23)和ORF10(p9)。系统发育分析将该病毒聚集在Olivirus属中,克龙病毒科,最接近的物种是橄榄病毒,俗称橄榄叶黄化相关病毒(OLYaV)。然而,所有分类学相关蛋白质的氨基酸序列显示,在所有情况下,OLYaV和新病毒之间的差异高于25%,表明它代表了Olivirus属中的一个新物种,为此提出了橄榄叶斑驳病毒(OLMV)的通用名称。这项研究代表了橄榄病毒属的进步,并为橄榄病毒提供了新的见解。
Studies of the virome of
olive trees with symptoms of leaf mottling by high-throughput sequencing (HTS) revealed the presence of a new virus. Full coding genome sequences of two isolates were determined and consisted of a single RNA segment of 16,516 nt and 16,489, respectively. The genomic organization contained 10 open reading frames (ORFs) from 5\' to 3\': ORF1a, ORF1b (RdRp), ORF2 (p22), ORF3 (p7), ORF4 (HSP70h), ORF5 (HSP90h), ORF6 (CP), ORF7 (p19), ORF8 (p12), ORF9 (p23) and ORF10 (p9). Phylogenetic analyses clustered this virus in the genus Olivavirus, family Closteroviridae, with the closest species being Olivavirus flaviolae, commonly named
olive leaf yellowing-associated virus (OLYaV). However, amino acid sequences of all taxonomically relevant proteins showed, in all cases, a divergence higher than 25% between OLYaV and the new virus, indicating that it represents a new species in the genus Olivavirus for which the common name of
olive leaf mottling virus (OLMV) is proposed. This study represents an advance in the genus Olivavirus and provides new insights into the
olive virome.