■哮喘的特征在于肺细胞浸润和气道的高反应性。短期压力减少气道炎症。因此,在本研究中,我们研究了反复注射生理盐水引起的短期应激对大鼠肺过敏性炎症反应的影响。
■成年雄性大鼠分为三组:未致敏组(未致敏,挑战,或治疗过的大鼠),对照组(大鼠以卵清蛋白(OVA)致敏诱导肺部炎症),和盐水组(OVA致敏前用盐水处理五天的大鼠)。在助推器后一周用雾化OVA进行吸入挑战。第18天,生理盐水注射对支气管肺泡灌洗(BAL)中总白细胞和差异白细胞的影响,股骨髓灌洗(FML),并对血液进行了评估。粘液的百分比,血清皮质酮,胶原蛋白,肺外植体中的细胞因子,还测量了去甲肾上腺素水平。
■OVA致敏增加了循环白细胞及其向肺的迁移,减少骨髓白细胞。重复的盐水注射通过减少对照组中BAL和血液中白细胞的数量来防止这种迁移。细胞因子白细胞介素-4(IL-4)在对照组中高于幼稚组和生理盐水组;细胞因子白细胞介素-6(IL-6),白细胞介素-10(IL-10),对照组和生理盐水组的肿瘤坏死因子-α(TNFα)高于未治疗组;生理盐水组的干扰素γ(IFNγ)高于未治疗组和对照组;去甲肾上腺素在OVA致敏的动物中增加,仅在生理盐水组中高于未治疗组。
■这些结果表明,短期压力可能有助于给定治疗的抗过敏气道炎症作用。
UNASSIGNED: Asthma is characterized by pulmonary cell infiltration and hyper-responsiveness of the airways. Short-term stress reduces airway inflammation. Thus, in the present study, we examined the effects of short-term stress induced by repeated treatment with saline injections on the pulmonary allergic inflammatory response in rats.
UNASSIGNED: Adult male rats were divided into three groups: Naïve group (non-sensitized, challenged, or treated rats), Control group (rats sensitized with ovalbumin (OVA) to induce lung inflammation), and Saline group (rats treated for five days with saline before OVA sensitization). Inhalation challenges were performed one week after the booster with aerosolized OVA. On day 18, the effect of saline injections on total and differential leukocytes in bronchoalveolar lavage (BAL), femoral marrow lavage (FML), and blood was evaluated. The percentage of mucus, serum corticosterone, collagen, cytokines in lung explants, and norepinephrine levels were also measured.
UNASSIGNED: OVA sensitization increased the circulating leukocytes and their migration to the lung, decreasing the bone marrow leukocytes. The repeated saline injections prevented this migration by decreasing the number of leukocytes in BAL and blood in the control group. Cytokine Interleukin-4 (IL-4) was higher in the control group than in the naive and saline groups; cytokines Interleukin-6 (IL-6), Interleukin-10 (IL-10), and tumor necrosis factor-alpha (TNFα) were higher in the control and saline groups than in the naïve group; Interferon gamma (IFNγ) was higher in the saline group than in the naive and control groups; norepinephrine increased in animals sensitized with OVA and was higher only in the saline group relative to the naïve group.
UNASSIGNED: These results suggest that short-term stress could contribute to the anti-allergic airway inflammation effects of a given treatment.