有几组病毒,包括昆虫特异性病毒(ISV),如分类单元网状病毒,一组与植物病毒系统发育相关的病毒。网状病毒在蚊子细胞中复制,但不是在脊椎动物细胞中。
将食血节肢动物池接种在Vero和C6/36细胞中。观察细胞以检测可能的细胞病变效应。然后,间接免疫荧光,RT-PCR,并进行核苷酸测序。
对黄病毒呈阴性结果的七个样本,甲病毒和布尼亚病毒,但在C6/36细胞中显示出细胞病变效应。我们确定了各种ISV的发生,其中大多数属于分类单元Negesirus:Brejeira,内盖夫,科尔多瓦和沃勒菲尔德病毒,包括一种新的科学病毒,暂时命名为费托萨病毒。
我们在Amazon区域中检测到Negevirus,包括在巴西首次分离出的两种病毒:科尔多瓦病毒和内盖夫病毒,一种新的科学病毒:Feitosa病毒。
There are several groups of viruses including Insect Specific Viruses (ISV) such as the taxon
Negevirus, a group of viruses phylogenetically related to plant viruses. Negeviruses replicate in mosquito cells, but not in vertebrate cells.
Pools of hematophagous arthropods were inoculated in Vero and C6/36 cells. The cells were observed to detect possible cytopathic effect. Then, indirect immunofluorescence, RT-PCR, and nucleotide sequencing were performed.
Seven samples which presented negative results for flaviviruses, alphaviruses and bunyaviruses, but showed cytopathic effect in C6/36 cells were sequenced. We identified the occurrence of a variety of ISVs, most of them belonging to the taxon
Negevirus: The Brejeira, Negev, Cordoba and Wallerfield viruses, including a new virus for science, tentatively named Feitosa virus.
We detected negeviruses in the Amazon region, including two viruses that were isolated for the first time in Brazil: Cordoba virus and the Negev virus and, a new virus for science: the Feitosa virus.