PDF(Pubmed)
Abstract：
Advances in sequencing technologies and bioinformatics have greatly enhanced our knowledge of virus biodiversity. Currently, the viromes of hematophagous invertebrates, such as mosquitoes and ixodid ticks, are being actively studied. Tabanidae (Diptera) are a widespread family, with members mostly known for their persistent hematophagous behavior. They transmit viral, bacterial, and other pathogens, both biologically and mechanically. However, tabanid viromes remain severely understudied. In this study, we used high-throughput sequencing to describe the viromes of several species in the Hybomitra, Tabanus, Chrysops, and Haematopota genera, which were collected in two distant parts of Russia: the Primorye Territory and Ryazan Region. We assembled fourteen full coding genomes of novel viruses, four partial coding genomes, as well as several fragmented viral sequences, which presumably belong to another twelve new viruses. All the discovered viruses were tested for their ability to replicate in mammalian porcine embryo kidney (PEK), tick HAE/CTVM8, and mosquito C6/36 cell lines. In total, 16 viruses were detected in at least one cell culture after three passages (for PEK and C6/36) or 3 weeks of persistence in HAE/CTVM8. However, in the majority of cases, qPCR showed a decline in virus load over time.
摘要：
测序技术和生物信息学的进步极大地增强了我们对病毒生物多样性的了解。目前,嗜血无脊椎动物的病毒体，比如蚊子和ixodid蜱，正在积极研究。Tabanidae（双翅目）是一个广泛的家庭，成员大多以持续的吸血行为而闻名。它们传播病毒，细菌,和其他病原体，生物和机械。然而,塔巴尼德病毒仍然被严重低估。在这项研究中,我们使用高通量测序来描述Hybomitra中几种病毒的类型，Tabanus,菊花,和Haematopota属，收集在俄罗斯的两个遥远的地区：滨海边疆区和梁赞地区。我们组装了十四个新病毒的完整编码基因组，四个部分编码基因组，以及几个片段化的病毒序列，大概属于另外十二种新病毒。测试了所有发现的病毒在哺乳动物猪胚胎肾（PEK）中的复制能力，蜱HAE/CTVM8和蚊子C6/36细胞系。总的来说,在HAE/CTVM8中三代(对于PEK和C6/36)或持续3周后,在至少一种细胞培养物中检测到16种病毒。然而,在大多数情况下，qPCR显示病毒载量随时间下降。
