老花眼是一个全球性问题,全世界估计有13亿患者。在功能食品应用领域,膳食补充剂或草药,很少有报告描述其使用的积极影响。在现有文献中,缺乏对人体和动物模型的提取物的研究,同时,来自多酚基团的化合物(特别是,花青素)和环烯醚萜类,因此,我们对由这些化合物组成的制剂对视觉器官状况的影响进行了研究。我们以前在兔子模型上的经验证明了口服山茱萸提取物对稳定眼内压的积极作用。这项研究的目的是评估口服给药的三元复合制剂对眼器官生理参数状态的影响。该制剂含有苦莓黑果的提取物,金银花忍冬,和越橘越橘(以下简称AKB)标准化为花青素和环烯醚萜类,作为文献中已知的生物活性化合物。一个随机的,双盲,为期17周的“冲洗期”的交叉研究评估了一组23名50岁以上的人,他们患有老花眼,在屏幕监视器前长时间工作。这组志愿者是从每天从事白领工作的人中招募的。制剂中所含试验物质对远近视敏度的影响,距离和距离的对比感,眼内压,和结膜润滑,通过Schirmer测试,LIPCOF指数和TBUT测试,和视野测试进行了评估。花青素(包括花青素3-O-半乳糖苷,甲酚3-O-阿拉伯糖苷,花青素3-O-葡萄糖苷,矢车菊苷3-O-rutinoside,矢车菊素3-O-阿拉伯糖苷)和环烯醚萜类(包括马根素,Sweroside,loganicacid)被鉴定为通过HPLC-MS获得的提取物中存在的物质。初步结果表明,口服施用的AKB组合物在给药的前6周没有改变视敏度。仅在研究的下一个周期中,在92.3%的患者中观察到近视敏度的改善。这可能表明纠正老花眼患者的近视力的潜力。另一方面,在给药第6周开始的Schirmer试验中,80%的患者观察到结膜润湿改善.在进行实验的随后几周中,该效应减弱至61.5%。Schirmer试验中结膜水合作用的改善显示了AKB制剂在一组干眼综合征患者中的潜在有益效果。这是第一个基于天然制剂的研究,苦莓的标准化提取物,金银花浆果,还有越橘.初步研究表明,在Schirmer测试中,近视敏度和结膜水合作用有所改善,但这需要在进一步的研究中得到证实。
Presbyopia is a global problem with an estimated 1.3 billion patients worldwide. In the area of functional food applications, dietary supplements or herbs, there are very few reports describing the positive effects of their use. In the available literature, there is a lack of studies in humans as well as on an animal model of extracts containing, simultaneously, compounds from the polyphenol group (in particular, anthocyanins) and iridoids, so we undertook a study of the effects of a preparation composed of these compounds on a condition of the organ of vision. Our previous experience on a rabbit model proved the positive effect of taking an oral extract of Cornus mas in stabilizing the intraocular pressure of the eye. The purpose of this study was to evaluate the effect of an orally administered ternary compound preparation on the status of physiological parameters of the ocular organ. The preparation contained an extract of the chokeberry Aronia melanocarpa, the honeysuckle berry Lonicera caerulea L., and the bilberry Vaccinium myrtillus (hereafter AKB) standardized for anthocyanins and iridoids, as bioactive compounds known from the literature. A randomized, double-blind, cross-over study lasting with a \"wash-out\" period of 17 weeks evaluated a group of 23 people over the age of 50, who were subjects with presbyopia and burdened by prolonged work in front of screen monitors. The group of volunteers was recruited from people who perform white-collar jobs on a daily basis. The effects of the test substances contained in the preparation on visual acuity for distance and near, sense of contrast for distance and near, intraocular pressure, and conjunctival lubrication, tested by Schirmer test, LIPCOF index and TBUT test, and visual field test were evaluated. Anthocyanins (including cyanidin 3-O-galactoside, delphinidin 3-O-arabinoside, cyanidin 3-O-glucoside, cyanidin 3-O-rutinoside, cyanidin 3-O-arabinoside) and iridoids (including loganin, sweroside, loganic acid) were identified as substances present in the extract obtained by HPLC-MS. The preliminary results showed that the composition of AKB applied orally does not change visual acuity in the first 6 weeks of administration. Only in the next cycle of the study was an improvement in near visual acuity observed in 92.3% of the patients. This may indicate potential to correct near vision in presbyopic patients. On the other hand, an improvement in conjunctival wetting was observed in the Schirmer test at the beginning of week 6 of administration in 80% of patients. This effect was weakened in subsequent weeks of conducting the experiment to 61.5%. The improvement in conjunctival hydration in the Schirmer test shows the potential beneficial effect of the AKB formulation in a group of patients with dry eye syndrome. This is the first study of a preparation based on natural, standardized extracts of chokeberry, honeysuckle berry, and bilberry. Preliminary studies show an improvement in near visual acuity and conjunctival hydration on the Schirmer test, but this needs to be confirmed in further studies.