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Abstract：
The present work aims to study the possibilities of developing silver nanoparticles using natural extracts of grape pomace wastes originating from the native variety of Fetească Neagră 6 Șt. This study focused on investigating the influence of grape pomace extract obtained by two different extraction methods (classical temperature extraction and microwave-assisted extraction) in the phytosynthesis process of metal nanoparticles. The total phenolic content of the extracts was assessed using the spectrophotometric method with the Folin-Ciocâlteu reagent, while the identification and quantification of specific components were conducted through high-performance liquid chromatography with a diode array detector (HPLC-DAD). The obtained nanoparticles were characterized by UV-Vis absorption spectroscopy, X-ray diffraction (XRD), and transmission electron microscopy (TEM), along with assessing their antioxidant and antimicrobial properties against Gram-positive bacteria. The data collected from the experiments indicated that the nanoparticles were formed in a relatively short period of time (96 h) and, for the experimental variant involving the use of a 1:1 ratio (v/v, grape pomace extract: silver nitrate) for the nanoparticle phytosynthesis, the smallest crystallite sizes (from X-ray diffraction-4.58 nm and 5.14 nm) as well as spherical or semispherical nanoparticles with the lowest average diameters were obtained (19.99-23 nm, from TEM analysis). The phytosynthesis process was shown to enhance the antioxidant properties (determined using the DPPH assay) and the antimicrobial potential (tested against Gram-positive strains) of the nanoparticles, as evidenced by comparing their properties with those of the parent extracts; at the same time, the nanoparticles exhibited a selectivity in action, being active against the Staphylococcus aureus strain while presenting no antimicrobial potential against the Enterococcus faecalis strain.
摘要：
本工作旨在研究使用源自Feteasc边沿Neagr边沿6t天然品种的葡萄果渣废物的天然提取物开发银纳米颗粒的可能性。这项研究的重点是研究通过两种不同的提取方法（经典温度提取和微波辅助提取）获得的葡萄果渣提取物在金属纳米颗粒植物合成过程中的影响。提取物的总酚含量是使用分光光度法用Folin-Ciocalteu试剂评估的，同时通过具有二极管阵列检测器（HPLC-DAD）的高效液相色谱法对特定成分进行鉴定和定量。通过紫外-可见吸收光谱对所获得的纳米粒子进行了表征,X射线衍射(XRD)和透射电子显微镜（TEM），以及评估它们对革兰氏阳性细菌的抗氧化和抗菌性能。从实验中收集的数据表明，纳米颗粒是在相对较短的时间内（96小时）形成的，对于涉及使用1：1比例(v/v，葡萄果渣提取物：硝酸银）用于纳米颗粒植物合成，获得了最小的微晶尺寸（来自X射线衍射-4.58nm和5.14nm）以及具有最低平均直径的球形或半球形纳米颗粒（19.99-23nm，来自TEM分析)。植物合成过程显示出增强纳米颗粒的抗氧化特性（使用DPPH测定法确定）和抗微生物潜力（针对革兰氏阳性菌株进行测试），通过将它们的属性与母体提取物的属性进行比较可以证明；同时，纳米粒子在行动中表现出选择性，对金黄色葡萄球菌菌株具有活性，而对粪肠球菌菌株没有抗菌潜力。
