许多危险的细菌已经对传统抗生素产生了高度耐药性,这是一个巨大的公共卫生问题。这项研究调查了在嗜酸乳杆菌的培养滤液中生物合成的银纳米颗粒作为抗菌剂的用途。紫外可见分光光度法,傅里叶变换红外光谱,X射线粉末衍射,和扫描电子显微镜都验证了这些发现。生物合成的纳米颗粒的尺寸范围为33至90nm。然后使用9只分为三组的200gBW大鼠研究所产生的纳米银的细胞毒性。与对照组相比,经治疗的大鼠几乎没有毒性迹象;生理功能参数,包括丙氨酸转氨酶,天冬氨酸转氨酶,白蛋白,肌酐,和尿素在治疗和未治疗的动物中显著不同。此外,在多药耐药(MDR)病原菌中检查了生成的银纳米颗粒的抗菌作用,普通变形杆菌,大肠杆菌,金黄色葡萄球菌,和肺炎克雷伯菌,揭示了对所检查的细菌的高抗菌活性。为了进一步证明纳米银对处理和未处理细菌的转录和基因调控的影响,使用了差异显示液滴数字PCR,结果显示有几个基因被上调和下调。选择一些基因进行DNA测序,并根据序列分析,这些基因是mecA,β-内酰胺,和未知的蛋白质基因,这些已被保存在GenBank数据库中,登录号如下:葡萄球菌MZ748472和克雷伯菌MZ748473。我们得出结论,嗜酸乳杆菌生物合成的银纳米颗粒是环境友好的,并且对MDR病原菌具有抗菌活性。
Many dangerous bacteria have become highly resistant to traditional antibiotics, which is a huge public health concern. This study investigated the use of silver nanoparticles biosynthesized in a culture filtrate of Lactobacillus acidophilus as antimicrobials. UV-visual spectrophotometry, Fourier-transform-infrared spectroscopy, X-ray power diffraction, and scanning electron microscopy have all validated the findings. The biosynthesized nanoparticles ranged in size from 33 to 90 nm. The cytotoxicity of the
nanosilver generated was then investigated using nine 200 g BW rats separated into three groups. When compared to the control group, the treated rats showed little signs of toxicity; parameters of physiological function, including alanine transaminase, aspartate aminotransferase, albumin, creatinine, and urea were significantly different in treated and non-treated animals. Moreover, the antibacterial role of the generated silver nanoparticles was examined in multi-drug resistant (MDR) pathogenic bacteria, Proteus vulgaris, Escherichia coli, Staphylococcus aureus, and Klebsiella pneumoniae, revealing high antibacterial activity against the examined bacteria. For more demonstration of the effect of the
nanosilver on transcription and gene regulation of treated and non-treated bacteria differential display droplet digital-PCR was used, and the results revealed that several genes were up- and down-regulated. Some genes were selected for DNA sequencing and according to the sequence analysis, these genes were mecA, beta-lactam, and unidentified protein genes, and these have been deposited in the GenBank Database with the following accession numbers: Staphylococcus MZ748472 and Klebsiella MZ748473. We conclude that silver nanoparticles biosynthesized by L. acidophilus are environmentally friendly and have antibacterial activities against MDR pathogenic bacteria.