Abstract：
The development of novel implants subjected to surface modification to achieve high osteointegration properties at simultaneous antimicrobial activity is a highly current problem. This study involved different surface treatments of titanium surface, mainly by electrochemical oxidation to produce a nanotubular oxide layer (TNTs), a subsequent electrochemical reduction of silver nitrate and decoration of a nanotubular surface with silver nanoparticles (AgNPs), and finally electrophoretic deposition (EPD) of a composite of chitosan (CS) and either polymethacrylate-based copolymer Eudragit E 100 (EE100) or poly(4-vinylpyridine) (P4VP) coating. The effects of each stage of this multi-step modification were examined in terms of morphology, roughness, wettability, corrosion resistance, coating-substrate adhesion, antibacterial properties, and osteoblast cell adhesion and proliferation. The results showed that the titanium surface formed nanotubes (inner diameter of 97 ± 12 nm, length of 342 ± 36 nm) subsequently covered with silver nanoparticles (with a diameter of 88 ± 8 nm). Further, the silver-decorated nanotubes were tightly coated with biopolymer films. Most of the applied modifications increased both the roughness and the surface contact angle of the samples. The deposition of biopolymer coatings resulted in reduced burst release of silver. The coated samples revealed potent antimicrobial activity against both Gram-positive and Gram-negative bacteria. Total elimination (99.9%) of E. coli was recorded for a sample with CS/P4VP coating. Cytotoxicity results using hFOB 1.19, a human osteoblast cell line, showed that after 3 days the tested modifications did not affect the cellular growth according to the titanium control. The proposed innovative multilayer antibacterial coatings can be successful for titanium implants as effective postoperative anti-inflammation protection.
摘要：
进行表面改性以在同时具有抗微生物活性的情况下实现高骨整合特性的新型植入物的开发是当前的高度问题。这项研究涉及钛表面的不同表面处理，主要通过电化学氧化产生纳米管状氧化物层(TNTs)，随后，硝酸银的电化学还原和用银纳米颗粒（AgNP）装饰纳米管表面，最后电泳沉积(EPD)壳聚糖(CS)和基于聚甲基丙烯酸酯的共聚物EudragitE100(EE100)或聚(4-乙烯基吡啶)(P4VP)涂层的复合材料。在形态方面检查了这种多步骤改性的每个阶段的影响，粗糙度,润湿性,耐腐蚀性，涂层-基材附着力，抗菌性能，成骨细胞的粘附和增殖。结果表明，钛表面形成的纳米管（内径为97±12nm，342±36nm的长度)随后用银纳米颗粒(具有88±8nm的直径)覆盖。Further,银修饰的纳米管紧密地涂覆有生物聚合物膜。大多数应用的改性增加了样品的粗糙度和表面接触角。生物聚合物涂层的沉积导致银的突释减少。包被的样品显示出针对革兰氏阳性和革兰氏阴性细菌的有效抗微生物活性。对于具有CS/P4VP涂层的样品,记录大肠杆菌的完全消除(99.9%)。使用人成骨细胞系hFOB1.19的细胞毒性结果，显示3天后,根据钛对照,测试的修饰不影响细胞生长。所提出的创新多层抗菌涂层可以成功用于钛植入物作为有效的术后抗炎保护。
