简介:乳腺癌是全球女性死亡的重要原因,目前的治疗方法由于副作用和耐药性而面临挑战。纳米技术通过实现靶向药物递送和最小化对正常组织的毒性提供有前景的解决方案。方法:在本研究中,我们开发了一个名为(Alg-AgNPs-CisPt)的复合平台,由包埋顺铂的藻酸盐水凝胶涂覆的银纳米颗粒组成。我们检查了这种纳米复合物在诱导对乳腺癌细胞的协同细胞毒性作用中的有效性。结果和讨论:使用各种分析技术的表征证实了纳米复合物的组成及其组分的分布。细胞毒性测定和细胞凋亡分析表明,与作为独立治疗的AgNP或顺铂相比,纳米复合物对乳腺癌细胞表现出更大的功效。此外,纳米复合物被发现可以增强细胞内活性氧的水平,进一步验证其功效。纳米复合物成分的协同作用在减少与作为独立治疗的较高剂量的顺铂相关的副作用方面提供了潜在的优势。总的来说,这项研究强调了(Alg-AgNPs-CisPt)纳米复合物作为一种有前途的平台嵌入成分的潜力,对乳腺癌细胞具有协同作用。
Introduction: Breast cancer is a significant cause of mortality in women globally, and current treatment approaches face challenges due to side effects and drug resistance. Nanotechnology offers promising solutions by enabling targeted drug delivery and minimizing toxicity to normal tissues. Methods: In this study, we developed a composite platform called (Alg-AgNPs-CisPt), consisting of silver nanoparticles coated with an alginate hydrogel embedding cisplatin. We examined the effectiveness of this
nanocomplex in induce synergistic cytotoxic effects on breast cancer cells. Results and Discussion: Characterization using various analytical techniques confirmed the composition of the nanocomplex and the distribution of its components. Cytotoxicity assays and apoptosis analysis demonstrated that the
nanocomplex exhibited greater efficacy against breast cancer cells compared to AgNPs or cisplatin as standalone treatments. Moreover, the
nanocomplex was found to enhance intracellular reactive oxygen species levels, further validating its efficacy. The synergistic action of the
nanocomplex constituents offers potential advantages in reducing side effects associated with higher doses of cisplatin as a standalone treatment. Overall, this study highlights the potential of the (Alg-AgNPs-CisPt)
nanocomplex as a promising platform embedding components with synergistic action against breast cancer cells.