Abstract：
RNA interference (RNAi)-based biopesticides offer an attractive avenue for pest control. Previous studies revealed high RNAi sensitivity in Holotrichia parallela larvae, showcasing its potential for grub control. In this study, we aimed to develop an environmentally friendly RNAi method for H. parallela larvae. The double-stranded RNA (dsRNA) of the V-ATPase-a gene (HpVAA) was loaded onto layered double hydroxide (LDH). The dsRNA/LDH nanocomplex exhibited increased environmental stability, and we investigated the absorption rate and permeability of dsRNA-nanoparticle complexes and explored the RNAi controlling effect. Silencing the HpVAA gene was found to darken the epidermis of H. parallela larvae, with growth cessation or death or mortality, disrupting the epidermis and midgut structure. Quantitative reverse transcription-polymerase chain reaction and confocal microscopy confirmed the effective absorption of the dsRNA/LDH nanocomplex by peanut plants, with distribution in roots, stems, and leaves. Nanomaterial-mediated RNAi silenced the target genes, leading to the death of pests. Therefore, these findings indicate the successful application of the nanomaterial-mediated RNAi system for underground pests, thus establishing a theoretical foundation for developing a green, safe, and efficient pest control strategy.
摘要：
基于RNA干扰(RNAi)的生物农药为害虫控制提供了有吸引力的途径。先前的研究表明，在平行Holotrichia幼虫中具有很高的RNAi敏感性，展示其在grub控制方面的潜力。在这项研究中,我们的目的是开发一种环境友好的RNA干扰方法。将V-ATP酶-a基因(HpVAA)的双链RNA(dsRNA)加载到层状双氢氧化物(LDH)上。dsRNA/LDH纳米复合物表现出增加的环境稳定性，我们研究了dsRNA-纳米颗粒复合物的吸收率和通透性，并探索了RNAi的控制作用。发现沉默HpVAA基因会使H.parliela幼虫的表皮变暗，生长停止或死亡或死亡，破坏表皮和中肠结构。定量逆转录-聚合酶链反应和共聚焦显微镜证实了花生植物对dsRNA/LDH纳米复合物的有效吸收，在根中分布，茎,和树叶。纳米材料介导的RNAi沉默了靶基因，导致害虫死亡。因此,这些发现表明纳米材料介导的RNAi系统在地下害虫中的成功应用，从而为发展绿色，安全,和有效的害虫控制策略。
