油菜花物种作为观赏植物有多种用途,食物,和医学。然而,他们的基因组信息是有限的;I.alba和I.obscura被测序和组装。它们的叶绿体基因组分别为161,353bp和159,691bp,分别。两个基因组都表现出四方结构,由一对反向重复(IR)区域组成,它们由大型单拷贝(LSC)和小型单拷贝(SSC)区域分隔。两个基因组的总GC含量为37.5%。共有104个和93个简单序列重复,50个大重复,在两个叶绿体基因组中鉴定出30和22个短串联重复序列,分别。根据奇偶校验规则2图分析,在第三碱基位置,G和T比C和A更优选。中性图显示相关系数为0.126和0.105,表明自然选择对大多数蛋白质编码基因(CDS)的密码子使用偏好的影响。使用来自泰国的31个选定的Ipomoea分类群进行的基因组比较分析表明,它们的叶绿体基因组相当保守,但是在其中一些葡萄籽类群中发现了IR区域的扩张或收缩。共确定了五个高度分歧的区域,包括CDS基因,ndhA,和ndhF,以及基因间间隔区psbI-atpA和rpl32-ccsA。基于31个Ipomoea分类群的完整叶绿体基因组序列和CDS数据集的系统发育分析表明,I.alba被解析为系列的组成员(ser。)Quamoclit,其中包含其他七个分类单元,包括I.Hederacea,I.improati,I.indica,一、无,我是紫癜,I.quamoclit,还有I.Xsloteri,而I.obscura与I.tiliifolia分组,两者都在ser之下。Obscura,与ser的I.biflora密切相关。Pes-tigridis.使用完整的叶绿体基因组序列数据集进行的发散时间估计表明,Ipomoeeae的平均发散年龄,Argyreiinae,和Astripomoeinae,大约是29.99Mya,19.81Mya,和13.40Mya,分别。指示I.alba与Ipomoea其他成员的差异的节点约为10.06Mya,人们认为I.obscura和I.tiliifolia之间的分裂发生在17.13Mya左右。来自泰国和台湾的I.obscura加入之间的分歧被认为发生在0.86Mya左右。
Ipomoea species have diverse uses as ornamentals, food, and medicine. However, their genomic information is limited; I. alba and I. obscura were sequenced and assembled. Their chloroplast genomes were 161,353 bp and 159,691 bp, respectively. Both genomes exhibited a quadripartite structure, consisting of a pair of inverted repeat (IR) regions, which are separated by the large single-copy (LSC) and small single-copy (SSC) regions. The overall GC content was 37.5% for both genomes. A total of 104 and 93 simple sequence repeats, 50 large repeats, and 30 and 22 short tandem repeats were identified in the two chloroplast genomes, respectively. G and T were more preferred than C and A at the third base position based on the Parity Rule 2 plot analysis, and the neutrality plot revealed correlation coefficients of 0.126 and 0.105, indicating the influence of natural selection in shaping the codon usage bias in most protein-coding genes (CDS). Genome comparative analyses using 31 selected Ipomoea taxa from Thailand showed that their chloroplast genomes are rather conserved, but the presence of expansion or contraction of the IR region was identified in some of these Ipomoea taxa. A total of five highly divergent regions were identified, including the CDS genes accD, ndhA, and ndhF, as well as the intergenic spacer regions psbI-atpA and rpl32-ccsA. Phylogenetic analysis based on both the complete chloroplast genome sequence and CDS datasets of 31 Ipomoea taxa showed that I. alba is resolved as a group member for series (ser.) Quamoclit, which contains seven other taxa, including I. hederacea, I. imperati, I. indica, I. nil, I. purpurea, I. quamoclit, and I. × sloteri, while I. obscura is grouped with I. tiliifolia, both of which are under ser. Obscura, and is closely related to I. biflora of ser. Pes-tigridis. Divergence time estimation using the complete chloroplast genome sequence dataset indicated that the mean age of the divergence for Ipomoeeae, Argyreiinae, and Astripomoeinae, was approximately 29.99 Mya, 19.81 Mya, and 13.40 Mya, respectively. The node indicating the divergence of I. alba from the other members of Ipomoea was around 10.06 Mya, and the split between I. obscura and I. tiliifolia is thought to have happened around 17.13 Mya. The split between the I. obscura accessions from Thailand and Taiwan is thought to have taken place around 0.86 Mya.