疼痛的负面影响是动物手术管理中持续关注的问题,导致寻找新药或更有效的镇痛方案来控制这种负面情绪。这项研究旨在评估在异氟烷麻醉下进行选择性卵巢子宫切除术(OVH)的雌性犬中,单独使用大麻二酚(CBD)和美洛昔康联合使用红外瞳孔测定法的伤害性反应。包括总共60只不同品种的雌性狗。根据治疗将这些狗随机分配到四个研究组:接受盐水溶液的对照组(G0:n=15);预先用剂量为0.2mg/kg-1IV的美洛昔康给药的组(GMelox:n=15)。术后该药物每24小时以0.1mgKg-1IV使用;术前口服剂量为2mgKg-1的CBD治疗组(GCBD:n=15)。术后每12小时给药;该组术前服用美洛昔康和CBD(GMelox/CBD:n=15)美洛昔康联合用药,剂量为0.2mgKg-1IV,术后静脉注射0.1mgKg-1。术前口服2mgKg-1剂量的CBD,术后每12小时。术后48小时给予治疗。OVH之后,瞳孔神经指数,瞳孔大小,最小直径(MIN),百分比变化,收缩延迟(Lat),收缩速度,和最大收缩速度记录为事件期间双眼的瞳孔测量变量(E):基线(给药前30分钟),E30分钟,E1h,E2h,E3h,E4h,E8h,E12h,E24h,E48h格拉斯哥综合测量疼痛量表(GCMPS-SF)的简短形式用于评估相同事件期间的疼痛。总的来说,据观察,瞳孔测量变量大小,分钟.,还有Lat.在E30分钟内,G0显著高于其他组,E1h,和E2h(p=0.03),表明G0动物瞳孔扩张更大。此外,GCMPS-SF与GMelox之间无统计学差异,GCBD,和GMelox/CBD在术后期间(p>0.05)。相比之下,与G0相比,得分有统计学差异(p=0.00001),该组中的所有动物在手术后2小时接受抢救镇痛。根据瞳孔测量和GCMPS-SF量表的评分,据观察,大麻二酚单药治疗与美洛昔康单药或与大麻二酚联合治疗犬急性疼痛具有相似的镇痛效果.同样,这些发现表明,红外瞳孔测量可能是识别狗急性疼痛的工具。
The negative effects of pain are a constant concern in the surgical management of animals, leading to the search for new drugs or more effective analgesic protocols to control this negative emotion. This study aimed to evaluate the nociceptive response of cannabidiol (CBD) alone and in combination with
meloxicam using infrared pupillometry in female dogs undergoing elective ovariohysterectomy (OVH) under isoflurane anesthesia. A total of 60 female dogs of different breeds were included. These dogs were randomly assigned to four study groups according to the treatment: Control Group (G0: n = 15) receiving saline solution; group premedicated with
meloxicam at a dose of 0.2 mg Kg-1 IV (GMelox: n = 15). Postoperatively this drug was used at 0.1 mg Kg-1 IV every 24 h; the CBD-treated Group (GCBD: n = 15) at a dose of 2 mg Kg-1 orally in the preoperative. Postoperatively was administrated every 12 h; and the Group premedicated with the combination of
meloxicam and CBD (GMelox/CBD: n = 15)
Meloxicam at a dose of 0.2 mg Kg-1 IV preoperatively, and 0.1 mg Kg-1 IV during the postoperative. CBD at a dose of 2 mg Kg-1 orally in the preoperative, and every 12 h in the postoperative. Treatments were administered for 48 postoperative hours. After OVH, the pupillary neurologic index, pupillary size, minimum diameter (MIN), percentage change, constriction latency (Lat), constriction velocity, and maximum constriction velocity were recorded as pupillometric variables in both eyes during events (E): Baseline (30 min before drug administration), E30 min, E1h, E2h, E3h, E4h, E8h, E12h, E24h, and E48h. The Short-Form of the Glasgow Composite Measure Pain Scale (GCMPS-SF) was used to assess pain during the same events. Overall, it was observed that the pupillometric variables Size, MIN., and Lat. were significantly higher in G0 compared to the other groups during E30 min, E1h, and E2h (p = 0.03), indicating greater pupil dilation in G0 animals. Additionally, no statistically significant differences were observed in GCMPS-SF between GMelox, GCBD, and GMelox/CBD during the postoperative period (p > 0.05). In contrast, the scores were statistically different compared to G0 (p = 0.00001), where all animals in this group received rescue analgesia at 2 h post-surgery. According to pupillometry and scores on the GCMPS-SF scale, it was observed that monotherapy with cannabidiol provides a similar analgesic effect to
meloxicam alone or in combination with cannabidiol to manage acute pain in dogs. Similarly, these findings suggest that infrared pupillometry could be a tool for recognizing acute pain in dogs.