■自噬是允许通过溶酶体降解有害成分以在可变刺激下维持细胞稳态的过程。SQSTM1是参与功能性自噬的关键分子,与不同的信号通路相关。氧化反应,和炎症。自噬的失调被报道在广谱的疾病中。SQSTM1的积累反映了自噬受损,这与各种肿瘤的发生和进展有关,包括肝细胞癌(HCC)。这项研究调查了SQSTM1蛋白在HCC中的表达及其与临床病理特征的关系以及射频消融(RFA)后肿瘤复发的可能性。
■这项研究包括50例巴塞罗那诊所肝癌0/A-B期肝硬化HCC患者,符合RFA条件。就在局部消融之前获得肿瘤和肿瘤周围活检,并通过免疫组织化学评估肿瘤病理分级和SQSTM1表达。患者在完全消融后随访一年以检测任何肿瘤复发。
■血清甲胎蛋白水平(U=149.50,P=0.027*)和肿瘤病理分级(χ2=12.702,P=0.002*)与肿瘤对RFA的反应显着相关。SQSTM1表达水平显着增加,在肝癌与癌旁肝硬化肝组织相比(Z=5.927,P<0.001*)。SQSTM1在HCC中的表达水平与肿瘤的病理分级之间存在显着直接关系(H=33.789,P<0.001*)。关于后续行动,肿瘤和瘤周SQSTM1表达水平显着作为总生存率的潜在预测指标,但不是疾病复发。
■SQSTM1表达可以决定侵袭性肝癌,即使肿瘤大小和数量合理,并确定总生存期短和预后不良的HCC患者亚群。SQSTM1表达不能预测RFA后肝内HCC复发。SQSTM1可能是选择HCC患者进行未来治疗的潜在生物标志物和靶标。
UNASSIGNED: Autophagy is a process that allows the degradation of detrimental components through the lysosome to maintain cellular homeostasis under variable stimuli. SQSTM1 is a key molecule involved in functional autophagy and is linked to different signaling pathways, oxidative responses, and inflammation. Dysregulation of autophagy is reported in a broad spectrum of diseases. Accumulation of SQSTM1 reflects impaired autophagy, which is related to carcinogenesis and progression of various tumors, including hepatocellular carcinoma (HCC). This study investigated SQSTM1 protein expression in HCC and its relation to the clinicopathological features and the likelihood of tumor recurrence after radiofrequency ablation (RFA).
UNASSIGNED: This study included 50 patients with cirrhotic HCC of Barcelona Clinic Liver Cancer stages 0/A-B eligible for RFA. Tumor and peritumor biopsies were obtained just prior to local ablation and assessed for tumor pathological grade and SQSTM1 expression by immunohistochemistry. Patients were followed for one year after achieving complete ablation to detect any tumor recurrence.
UNASSIGNED: Serum alpha-fetoprotein level (U = 149.50, P = 0.027∗) and pathological grade of the tumor (χ2 = 12.702, P = 0.002∗) associated significantly with the tumor response to RFA. SQSTM1 expression level was significantly increased in HCC compared to the adjacent peritumor cirrhotic liver tissues (Z = 5.927, P < 0.001∗). Significant direct relation was found between SQSTM1 expression level in HCC and the pathological grade of the tumor (H = 33.789, P < 0.001∗). On follow-up, tumor and peritumor SQSTM1 expression levels performed significantly as a potential predictor of the overall survival, but not the disease recurrence.
UNASSIGNED: SQSTM1 expression could determine aggressive HCC, even with reasonable tumor size and number, and identify the subset of HCC patients with short overall survival and unfavorable prognosis. SQSTM1 expression could not predict post-RFA intrahepatic HCC recurrence. SQSTM1 may be a potential biomarker and target for the selection of HCC patients for future therapies.