背景:这项研究旨在利用拉合尔的叶表皮形态解剖特征确定15个唇科类群的分类位置和定界。该研究的主要目的还在于对巴基斯坦植物区系中的唇形科分类群进行修订和升级,因为在巴基斯坦的植物区系中没有发现研究物种的细节。
方法:检查重要的解剖参数,如表皮细胞的形状和大小,气孔类型,警卫和辅助细胞的形状和大小,气孔腔大小,毛状体的大小和形状,油滴,水晶,使用光学显微镜(LM)和扫描电子显微镜(SEM)技术研究了分泌腔特征。在所有研究过的唇形科物种中,这些解剖特征差异很大。进行了主成分分析和相关性分析,以区分物种相似性。
结果:大多数物种具有五边形和六边形的表皮细胞,具有直的背斜壁厚。在近轴曲面上,在罗勒和迷迭香中发现了副气孔。在Asugareptans中观察到了Diacytic气孔,在Galeopsis四打中观察到了类气孔。毛状体存在于五个物种中,即,Menthasuaveolens嗯。A.reptans,胸腺,M.单花萼,和SalviaSplendensEwat.在S.Splendens,看到了骨盆和腺毛,在其他物种中,毛状体长,不分枝的腺体,末端逐渐变细。在近轴侧毛状体仅存在于木瓜中,A.reptans,S.bazyntina,O.basciculum,S、辉煌,厚朴,S.迷迭香。在其他物种中,在正面没有毛状体。在背面视图中,M.suaveolens有最大的毛状体长度,O.basciculum有最小的。S.splendensL.具有最大的毛状体宽度,而T.vulgaris有最小的。
结论:因此,根据这些发现,形态解剖特征可用于鉴定唇形科类群。此外,有必要全面升级和增加巴基斯坦植物区系中研究的分类群。
BACKGROUND: This study was aimed to determine the taxonomic position and delimitation of fifteen Lamiaceae taxa using leaf epidermal morpho-anatomical features in Lahore. A main objective of the study was also the revision and upgradation of Lamiaceae taxa in the flora of Pakistan, as no details of studied species are found in the flora of Pakistan.
METHODS: The examination of significant anatomical parameters, such as epidermal cell shape and size, stomatal types, guard and subsidiary cells shape and size, stomatal cavity size, trichome size and shape, oil droplets, crystals, and secretory cavity characteristics were studied using light microscopic (LM) and scanning electron microscopic (SEM) techniques. Among all the studied Lamiaceae species, these anatomical features varied significantly. Principal component analysis and correlation were done to distinguish the species\' similarities.
RESULTS: Most species had pentagonal and hexagonal epidermal cells with straight anticlinal wall thickness. On the adaxial surface, paracytic stomata were found in Ocimum basilicum L. and Rosmarinus officinalis L. Diacytic stomata was observed in Ajuga reptans L. and anisocytic stomata in Galeopsis tetrahit L. In the abaxial surface, trichomes were present in five species, i.e., Mentha suaveolens Ehrh. A. reptans, Thymus vulgaris L., M. haplocalyx, and Salvia splendens Ewat. In S. splendens, peltate and glandular trichomes were seen whereas, in other species, trichomes were long, unbranched glandular and had tapering ends. In adaxial side trichomes were present only in M. suaveolens, A. reptans, S. bazyntina, O. basciculum, S. splendens, S. officinalis, S. rosemarinus. In other species, trichomes were absent on the adaxial surface. In abaxial view, M. suaveolens had the largest length of trichomes, and O. basciculum had the smallest. S. splendens L. had the largest trichome width, while T. vulgaris had the smallest.
CONCLUSIONS: Hence, according to these findings, morpho-anatomical traits are useful for identifying Lamiaceae taxa. Also, there is a need of upgradation and addition of studied taxa in flora of Pakistan comprehensively.