背景:一些证据表明弓形虫慢性感染(T。gondii)最近与人类和实验模型中的不孕症有关。这项基线研究旨在调查进入伊玛目霍梅尼医院体外受精(IVF)诊所的不孕妇女弓形虫感染的血清学证据。Mazandaran省,Sari,伊朗北部。
方法:在这项回顾性(描述性分析)研究中,所有不孕妇女在2010-2019年(10年)期间转诊到试管婴儿诊所,构成研究人群。包括人口统计学和一些相关特征在内的所有数据都收集到问卷中,并在Mazandaran医科大学的伊朗国家弓形虫病登记中心(INRCT)进行登记。伊朗北部。使用市售的酶联免疫吸附测定(ELISA)试剂盒(PishtazTeb,伊朗),基于制造商的协议。
结果:在520名不孕妇女中,反T.弓形虫IgG,在342/520中检测到IgM以及IgG和IgM抗体(65.77%),1/520(0.19)和4/520(0.77)不孕妇女,分别。IgG阳性不孕妇女中检出原发性和继发性不孕分别占74.56%和25.44%,分别。此外,大多数IgG血清阳性的受试者没有流产史,多囊卵巢综合征(PCO),纤维瘤,配偶使用避孕药和精索静脉曲张是不孕的主要原因。此外,抗弓形虫IgG不育妇女的血清催乳素和抗ullerian(AMH)激素水平在81.29和80.12%正常,分别。弓形虫感染的血清阳性率与这些与原发性不孕症相关的变量之间也存在统计学上的显着差异(P<0.05)。
结论:根据不育妇女中慢性弓形虫感染的高患病率(约三分之二),特别是那些有流产史和原发性不孕症的人,可以得出结论,潜在的弓形虫感染对研究区域的不孕妇女构成风险。因此,我们建议对不育症妇女进行弓形虫感染的筛查和治疗是有利的。
BACKGROUND: Some evidence reveled that chronic infection with Toxoplasma gondii (T. gondii) has recently been associated with infertility in human and experimental model. This baseline study aimed to investigate serological evidence of Toxoplasma infection among infertile women who admitted to the in vitro fertilization (IVF) clinic at Imam Khomeini Hospital, Mazandaran province, Sari, northern Iran.
METHODS: In this retrospective (descriptive-analytical) study, all infertile women referred to the IVF clinic during 2010-2019 (10 years), constitute the study population. All data including demographic and some related characteristics were collected into a questionnaire and registered at the Iranian National Registry Center for Toxoplasmosis (INRCT) at the Mazandaran University of Medical Sciences, northern Iran. The existence of anti-Toxoplasma antibodies (IgG and IgM) was explored using a commercially available enzyme-linked immune sorbent assay (ELISA) kit (PishtazTeb, Iran), based on the manufacturer\'s protocol.
RESULTS: Of 520 infertile women, anti-T. gondii IgG, IgM and both IgG and IgM antibodies were detected among 342/520 (65.77%), 1/520 (0.19) and 4/520 (0.77) infertile women, respectively. Primary and secondary infertility was detected in 74.56% and 25.44% of IgG seropositive infertile women, respectively. Also, most of the IgG seropositive subjects had no history of abortion, polycystic ovary syndrome (PCO), fibroma, contraceptive use and varicocele in spouse as primary cause of infertility. Furthermore, serum levels of prolactin and antimullerian (AMH) hormones were normal in 81.29 and 80.12% of infertile women with anti- T. gondii IgG, respectively. There was also a statistically significant difference between the seroprevalence of Toxoplasma infection and these variables associated to primary infertility (P < 0.05).
CONCLUSIONS: According to the high prevalence (about two thirds) of chronic T. gondii infection among infertile women, particularly those with a history of abortion and primary infertility, it can be concluded that latent Toxoplasma infection pose a risk to infertile woman in the study area. Therefore, we advise that screening and treatment of Toxoplasma infection among infertile women must be favorably considered.